Anti-P70 S6 Kinase beta antibody (ab70271)

Overview

  • Product name
    Anti-P70 S6 Kinase beta antibody
    See all P70 S6 Kinase beta primary antibodies
  • Description
    Rabbit polyclonal to P70 S6 Kinase beta
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between C terminal amino acids 430-482 of Human P70 S6 Kinase beta (NP_003943.2)

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
IP Use at 2-6 µg/mg of lysate.

Target

  • Function
    Phosphorylates specifically ribosomal protein S6.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa ribosomal protein S6 kinase 2 antibody
    • EC 2.7.11.1 antibody
    • KS6B2_HUMAN antibody
    • p70 beta antibody
    • p70 ribosomal S6 kinase beta antibody
    • p70 S6 kinase beta antibody
    • p70 S6K-beta antibody
    • p70 S6KB antibody
    • p70 S6Kbeta antibody
    • p70(S6K) beta antibody
    • p70-beta antibody
    • p70-S6K 2 antibody
    • P70S6K2 antibody
    • p70S6Kb antibody
    • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
    • Ribosomal protein S6 kinase B2 antibody
    • Ribosomal protein S6 kinase beta 2 antibody
    • Ribosomal protein S6 kinase beta-2 antibody
    • Rps6kb2 antibody
    • S6 kinase related kinase antibody
    • S6 kinase-related kinase antibody
    • S6K beta 2 antibody
    • S6K beta antibody
    • S6K-beta antibody
    • S6K-beta-2 antibody
    • S6K2 antibody
    • Serine/threonine protein kinase 14 beta antibody
    • Serine/threonine-protein kinase 14B antibody
    • SRK antibody
    • STK14B antibody
    see all

Images

  • All lanes : Anti-P70 S6 Kinase beta antibody (ab70271) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 15 µg
    Lane 2 : HeLa whole cell lysate at 5 µg


    Predicted band size : 53 kDa
    Observed band size : 53 kDa
    Additional bands at : 35 kDa,67 kDa,69 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds

References

ab70271 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for the additional protocol details and for the image provided. Have you tried any shorter incubations with the primary, such as 1 hr? Although we normally recommend ON for the strongest signal, reducing this time may eliminate some of the ba...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up