Overview

  • Product nameAnti-P70 S6 Kinase beta antibody
    See all P70 S6 Kinase beta primary antibodies
  • Description
    Mouse monoclonal to P70 S6 Kinase beta
  • Tested applicationsSuitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Partial recombinant RPS6KB2 protein fragment with the sequence

    MAAVFDLDLETEEGSEGEGEPELSPADACPLAELRAAGLEPVGHYEEVEL

    TETSVNVGPERIGPHSFELLRVLGKGGYGKVFQVRKVQGTNLGKIYAM KV and a proprietary tag.

  • Positive control
    • Transfected lysate (293T cells) and recombinant protein

Properties

Applications

Our Abpromise guarantee covers the use of ab76960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
ICC/IF Use a concentration of 10 µg/ml.
ELISA Use at an assay dependent concentration.

Target

  • FunctionPhosphorylates specifically ribosomal protein S6.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa ribosomal protein S6 kinase 2 antibody
    • EC 2.7.11.1 antibody
    • KS6B2_HUMAN antibody
    • p70 beta antibody
    • p70 ribosomal S6 kinase beta antibody
    • p70 S6 kinase beta antibody
    • p70 S6K-beta antibody
    • p70 S6KB antibody
    • p70 S6Kbeta antibody
    • p70(S6K) beta antibody
    • p70-beta antibody
    • p70-S6K 2 antibody
    • P70S6K2 antibody
    • p70S6Kb antibody
    • Ribosomal protein S6 kinase 70kDa, polypeptide 2 antibody
    • Ribosomal protein S6 kinase beta 2 antibody
    • Ribosomal protein S6 kinase beta-2 antibody
    • Rps6kb2 antibody
    • S6 kinase related kinase antibody
    • S6 kinase-related kinase antibody
    • S6K beta 2 antibody
    • S6K beta antibody
    • S6K-beta antibody
    • S6K-beta-2 antibody
    • S6K2 antibody
    • Serine/threonine protein kinase 14 beta antibody
    • Serine/threonine-protein kinase 14B antibody
    • SRK antibody
    • STK14B antibody
    see all

Anti-P70 S6 Kinase beta antibody images

  • Immunofluorescent analysis of HeLa cells with ab76960 at a concentration of 10 μg/mL.

  • Anti-P70 S6 Kinase beta antibody (ab76960) at 1/2500 dilution + Recombinant protein at 0.1 µg

    Predicted band size : 53 kDa

    This blot shows the antibody to be reactive against the recombinant protein used as the immunizing peptide (expected band size 37kDa).

  • All lanes : Anti-P70 S6 Kinase beta antibody (ab76960) at 1/1000 dilution

    Lane 1 : RPS6KB2 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 per lane.


    Predicted band size : 53 kDa
  • Detection limit for recombinant tagged RPS6KB2 (proprietary tag) is approximately 0.1ng/ml as a capture antibody.

References for Anti-P70 S6 Kinase beta antibody (ab76960)

ab76960 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"