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Rabbit polyclonal to P70 S6 Kinase beta (phospho T421 + S424)
Detects endogenous levels of Human p70 S6 Kinase beta only when phosphorylated at threonine 421 or serine 424. (The equivalent residues in other species are: Mouse: Thr444,Ser447; Rat: Thr421,Ser424)
Predicted to work with:
A synthetic phosphopeptide derived from human p70 S6 Kinase beta around the phosphorylation site of threonine 421 and serine 424(R-T
Extracts from NIH/3T3 cells, treated with EGF (200ng/ml, 30mins).
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 80 kDa.
Phosphorylates specifically ribosomal protein S6.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
70 kDa ribosomal protein S6 kinase 2 antibody
EC 18.104.22.168 antibody
Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody images
Western blot - P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029)
All lanes :
Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029) at 1/500 dilution
Lane 1 :
NIH 3T3 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Lane 2 :
NIH 3T3 cell extract treated with EGF (200ng/ml, 30mins)
Observed band size :
79 kDa (
why is the actual band size different from the predicted?
References for Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029)
has not yet been referenced specifically in any publications.
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