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Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 80 kDa.
Function Phosphorylates specifically ribosomal protein S6.
Sequence similarities Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain.
Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
70 kDa ribosomal protein S6 kinase 2 antibody
EC 22.214.171.124 antibody
Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody images
Western blot - P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029)
All lanes : Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029) at 1/500 dilution Lane 1 : NIH 3T3 cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide Lane 2 : NIH 3T3 cell extract treated with EGF (200ng/ml, 30mins) Observed band size : 79 kDa ( why is the actual band size different from the predicted?)
References for Anti-P70 S6 Kinase beta (phospho T421 + S424) antibody (ab53029)
has not yet been referenced specifically in any publications.
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