Overview

  • Product namep70S6K (pT389) PhosphoTracer ELISA KitSee all p70 S6 kinase (phospho Thr389) kits ...
  • Detection methodFluorescent
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture extracts
  • Assay typeSemi-quantitative
  • Sensitivity
    1 ng/ml
  • Range
    1 ng/ml - 300 ng/ml
  • Assay time
    2h 0m
  • Assay durationOne step assay
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.

    PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.

    A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.

     

    Abcam's PhosphoTracer p70S6K assay kits detect endogenous levels of p70S6K (GenBank Accession NP_003152) in cellular lysates. Phospho-p70S6K assay kits detect p70S6K only when phosphorylated at Thr389. Total p70S6K assay kits detect p70S6K, irrespective of phosphorylation status.



    The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionPhosphorylates specifically ribosomal protein S6.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Target information above from: UniProt accession Q9UBS0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • 70 kDa ribosomal protein S6 kinase 1
    • 70 kDa ribosomal protein S6 kinase 2
    • KLS
    • KS6B2_HUMAN
    • p70 alpha
    • P70 beta 1
    • P70 beta 2
    • p70 ribosomal S6 kinase beta
    • p70 S6 kinase alpha 1
    • p70 S6 kinase alpha 2
    • p70 S6 kinase beta
    • p70 S6K
    • p70 S6K alpha
    • p70 S6K-beta
    • p70 S6KA
    • p70 S6KB
    • p70(S6K) alpha
    • p70(S6K) beta
    • p70-beta
    • p70-S6K 2
    • P70S6K1
    • P70S6K2
    • p70S6Kb
    • PS6K
    • Ribosomal protein S6 kinase 70kDa polypeptide 1
    • Ribosomal protein S6 kinase 70kDa polypeptide 2
    • Ribosomal protein S6 kinase beta 1
    • Ribosomal protein S6 kinase beta-2
    • Ribosomal protein S6 kinase I
    • RPS6KB1
    • Rps6kb2
    • S6 kinase-related kinase
    • S6K
    • S6K beta 1
    • S6K-beta
    • S6K-beta-2
    • S6K1
    • S6K2
    • Serine/threonine kinase 14 alpha
    • Serine/threonine protein kinase 14 beta
    • Serine/threonine protein kinase 14A
    • Serine/threonine-protein kinase 14B
    • SRK
    • STK14A
    • STK14B
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab119642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration. Tested in MCF7, PC3.

p70S6K (pT389) PhosphoTracer ELISA Kit images

  • As shown, using the p70S6K assay kits, a significant reduction of p70S6K phosphorylation at Thr389 is detected in MCF7 cells treated with wortmannin for 3 hours, compared with untreated cells, while no change in total p70S6K levels was observed.
  • Using the PhosphoTracer phospho-p70S6K assay, the length of assay incubation time that was required to detect recombinant phospho-p70S6K was examined. After 15 mins, phospho- p70S6K concentrations of approximately 2 ng/mL were readily detected, while after 1 hour incubation, the limit of detection was approximately 0.5 ng/mL. Longer incubation times had little effect on overall limit of detection that was observed.
  • PhosphoTracer p70S6K (pT389) ELISA Kit (ab119642) used in Sandwich ELISA.

Protocols

References for p70S6K (pT389) PhosphoTracer ELISA Kit (ab119642)

ab119642 has not yet been referenced specifically in any publications.

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