p70S6K (pT389) PhosphoTracer ELISA Kit (ab119642)
- Product namep70S6K (pT389) PhosphoTracer ELISA KitSee all p70 S6 kinase (phospho Thr389) kits ...
- Detection methodFluorescent
- Tests1 x 96 well plate
- Sample typeCell culture extracts
- Assay typeSemi-quantitative
- Sensitivity1 ng/ml
- Range1 ng/ml - 300 ng/ml
- Assay time2h 0m
- Assay durationOne step assay
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Product overview
PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.
PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.
A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.
Abcam's PhosphoTracer p70S6K assay kits detect endogenous levels of p70S6K (GenBank Accession NP_003152) in cellular lysates. Phospho-p70S6K assay kits detect p70S6K only when phosphorylated at Thr389. Total p70S6K assay kits detect p70S6K, irrespective of phosphorylation status.
The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 96-well PhosphoTracer assay plate (stripwell) 1 unit Adherent plate seal 2 units ADHP (100X) 1 x 120µl ADHP Dilution Buffer 1 x 15ml Assay Control Lysate (lyophilized) 1 x 0.25ml Enhancer Solution 1 x 1ml Lysis Buffer (5X) 1 x 15ml Mouse monoclonal p70 S6 Kinase (HRP) (96 assay points) 1 x 3ml Rabbit monoclonal Phospho-p70 S6Kinase (Thr389) (96 assay points) 1 x 3ml Stop Solution 1 x 2ml Wash Buffer (10X) 1 x 15ml
- Research Areas
- FunctionPhosphorylates specifically ribosomal protein S6.
- Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- 70 kDa ribosomal protein S6 kinase 1
- 70 kDa ribosomal protein S6 kinase 2
- p70 alpha
- P70 beta 1
- P70 beta 2
- p70 ribosomal S6 kinase beta
- p70 S6 kinase alpha 1
- p70 S6 kinase alpha 2
- p70 S6 kinase beta
- p70 S6K
- p70 S6K alpha
- p70 S6K-beta
- p70 S6KA
- p70 S6KB
- p70(S6K) alpha
- p70(S6K) beta
- p70-S6K 2
- Ribosomal protein S6 kinase 70kDa polypeptide 1
- Ribosomal protein S6 kinase 70kDa polypeptide 2
- Ribosomal protein S6 kinase beta 1
- Ribosomal protein S6 kinase beta-2
- Ribosomal protein S6 kinase I
- S6 kinase-related kinase
- S6K beta 1
- Serine/threonine kinase 14 alpha
- Serine/threonine protein kinase 14 beta
- Serine/threonine protein kinase 14A
- Serine/threonine-protein kinase 14B
- Entrez Gene: 6199 Human
- Entrez Gene: 6198 Human
- Entrez Gene: 58988 Mouse
- Entrez Gene: 72508 Mouse
- Entrez Gene: 361696 Rat
- Entrez Gene: 83840 Rat
- Omim: 608939 Human
- Omim: 608938 Human
- SwissProt: Q9UBS0 Human
- SwissProt: Q9UBS0 Human
- SwissProt: Q9Z1M4 Mouse
- SwissProt: Q8BSK8 Mouse
- SwissProt: P67999 Rat
- Unigene: 534345 Human
Our Abpromise guarantee covers the use of ab119642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||sELISA. Tested in MCF7, PC3.|
p70S6K (pT389) PhosphoTracer ELISA Kit images
As shown, using the p70S6K assay kits, a significant reduction of p70S6K phosphorylation at Thr389 is detected in MCF7 cells treated with wortmannin for 3 hours, compared with untreated cells, while no change in total p70S6K levels was observed.
Using the PhosphoTracer phospho-p70S6K assay, the length of assay incubation time that was required to detect recombinant phospho-p70S6K was examined. After 15 mins, phospho- p70S6K concentrations of approximately 2 ng/mL were readily detected, while after 1 hour incubation, the limit of detection was approximately 0.5 ng/mL. Longer incubation times had little effect on overall limit of detection that was observed.
PhosphoTracer p70S6K (pT389) ELISA Kit (ab119642) used in Sandwich ELISA.
References for p70S6K (pT389) PhosphoTracer ELISA Kit (ab119642)
ab119642 has not yet been referenced specifically in any publications.