Recombinant Anti-p73 antibody [EP436Y] (ab40658)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP436Y] to p73
- Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-p73 antibody [EP436Y]
See all p73 primary antibodies -
Description
Rabbit monoclonal [EP436Y] to p73 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), ICC/IF, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Jurkat, HEK293 and NIH/3T3 cell lysates. IHC-P: Human urinary bladder carcinoma, human kidney, human liver carcinoma and mouse testis tissues. ICC/IF: HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP436Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 70 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
1/150 - 1/300.
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/500 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 70 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
1/150 - 1/300. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein. -
Tissue specificity
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue. -
Sequence similarities
Belongs to the p53 family.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
The WW-binding motif mediates interaction with WWOX. -
Post-translational
modificationsIsoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
Ubiquitinated; leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Accumulates in the nucleus in response to DNA damage. - Information by UniProt
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Database links
- Entrez Gene: 7161 Human
- Entrez Gene: 22062 Mouse
- Omim: 601990 Human
- SwissProt: O15350 Human
- SwissProt: Q9JJP2 Mouse
- Unigene: 697294 Human
- Unigene: 706990 Human
- Unigene: 78015 Mouse
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Alternative names
- p53 like transcription factor antibody
- p53 related protein antibody
- p53-like transcription factor antibody
see all
Images
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All lanes : Anti-p73 antibody [EP436Y] (ab40658) at 1/5000 dilution (purified)
Lane 1 : HeLa whole cell lysate
Lane 2 : HEK293 whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 60,75 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST. -
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling p73 with purified ab40658 at a dilution of 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Intracellular Flow Cytometry analysis of 293 (human embryonic kidney) cells labeling p73 with purified ab40658 at 1/120 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
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Anti-p73 antibody [EP436Y] (ab40658) at 1/1000 dilution (purified) + NIH/3T3 whole cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Blocking and dilution buffer: 5% NFDM/TBST. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urinary bladder carcinoma tissue labelling p73 with unpurified ab40658 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-p73 antibody [EP436Y] (ab40658) at 1/2000 dilution (unpurified)
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (58)
ab40658 has been referenced in 58 publications.
- Florian AC et al. Synergistic action of WDR5 and HDM2 inhibitors in SMARCB1-deficient cancer cells. NAR Cancer 4:zcac007 (2022). PubMed: 35252869
- Li Q et al. Disruption of GMNC-MCIDAS multiciliogenesis program is critical in choroid plexus carcinoma development. Cell Death Differ 29:1596-1610 (2022). PubMed: 35322202
- Ahrari A et al. Tetrodotoxin prevents heat-shock induced granule cell dispersion in hippocampal slice cultures. Front Cell Dev Biol 10:906262 (2022). PubMed: 36092698
- Maeso-Alonso L et al. p73 is required for vessel integrity controlling endothelial junctional dynamics through Angiomotin. Cell Mol Life Sci 79:535 (2022). PubMed: 36180740
- Sadžak A et al. Neurotoxic Effect of Flavonol Myricetin in the Presence of Excess Copper. Molecules 26:N/A (2021). PubMed: 33562817