Overview

  • Product nameAnti-p73 antibody - ChIP Grade
    See all p73 primary antibodies
  • Description
    Rabbit polyclonal to p73 - ChIP Grade
  • SpecificityThe antibody is not expected to react with deltaNp73.
  • Tested applicationsSuitable for: WB, IP, IHC-P, ChIPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chimpanzee, Rhesus monkey, Gorilla, African Green Monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human p73 aa 1-62. The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73.
    Database link: O15350

  • Positive control
    • 293T, MCF7 and COS-1 cells.
  • General notesCentrifuge tube to remove product from lid. This antibody has ben tested in ChIP at an inducible promotor held on a vector. There was very siginifant enrichment following induction at the locus analysed. This antibody works well in ChIP in this assay.

Properties

Applications

Our Abpromise guarantee covers the use of ab14430 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa).
IP Use at 1-4 µg/mg of lysate.
IHC-P Use at an assay dependent concentration. PubMed: 16630058
ChIP Use at an assay dependent concentration. PubMed: 21459846

Target

  • FunctionParticipates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
  • Tissue specificityExpressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
  • Sequence similaritiesBelongs to the p53 family.
    Contains 1 SAM (sterile alpha motif) domain.
  • DomainPossesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
    The WW-binding motif mediates interaction with WWOX.
  • Post-translational
    modifications
    Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
    Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
    Ubiquitinated; leading to its degradation by the proteasome.
  • Cellular localizationNucleus. Accumulates in the nucleus in response to DNA damage.
  • Information by UniProt
  • Database links
  • Alternative names
    • p53 like transcription factor antibody
    • p53 related protein antibody
    • p53-like transcription factor antibody
    • p53-related protein antibody
    • p73 antibody
    • P73_HUMAN antibody
    • TP73 antibody
    • Tumor protein p73 antibody
    see all

References for Anti-p73 antibody - ChIP Grade (ab14430)

This product has been referenced in:
  • Ratovitski EA Tumor Protein (TP)-p53 Members as Regulators of Autophagy in Tumor Cells upon Marine Drug Exposure. Mar Drugs 14:N/A (2016). Read more (PubMed: 27537898) »
  • Weilbacher A  et al. RITA can induce cell death in p53-defective cells independently of p53 function via activation of JNK/SAPK and p38. Cell Death Dis 5:e1318 (2014). WB . Read more (PubMed: 25010984) »

See all 6 Publications for this product

Product Wall

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Mouse Cell lysate - nuclear (mouse embrionic stem cell)
Specification mouse embrionic stem cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jul 02 2013

Thank you for contacting us.

Checking the datasheet, we have a positive Abreviews in which the customers used human choroid plexus lysateand cerebrospinal fluid in both ICC and WB:

http://www.abcam.com/index.html?datasheet=14430&am...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - nuclear (human cancer cell lines)
Specification human cancer cell lines
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Real-time PCR
Positive control stable Saos cell line with inducible overexpression of TAp73alpha and Tap73beta
Negative control Saos parental cells and Saos cells overexpressing p53
Username

Mrs. Daniela Kramer

Verified customer

Submitted May 03 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (CSF)
Loading amount 50 µg
Specification CSF
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Alfredo Cabrera

Verified customer

Submitted Dec 11 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"