Human Predicted to work with:
Mouse, Chimpanzee, Rhesus monkey, Gorilla, African green monkey, Orangutan
Synthetic peptide corresponding to Human p73 aa 1-62. The epitope is found in isoforms alpha, beta, gamma, delta, epsilon, zeta, and kappa of human p73. Database link: O15350
293T, MCF7 and COS-1 cells.
Centrifuge tube to remove product from lid.
This antibody has ben tested in ChIP at an inducible promotor held on a vector. There was very siginifant enrichment following induction at the locus analysed. This antibody works well in ChIP in this assay.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 70 kDa).
Use at 1-4 µg/mg of lysate.
Use at an assay dependent concentration. PubMed: 16630058
Use at an assay dependent concentration. PubMed: 21459846
Participates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
Expressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
Belongs to the p53 family. Contains 1 SAM (sterile alpha motif) domain.
Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain. The WW-binding motif mediates interaction with WWOX.
Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity. Higher levels of phosphorylation seen in the brain from patients with Huntington disease. Ubiquitinated; leading to its degradation by the proteasome.
Nucleus. Accumulates in the nucleus in response to DNA damage.
Ratovitski EA Tumor Protein (TP)-p53 Members as Regulators of Autophagy in Tumor Cells upon Marine Drug Exposure. Mar Drugs14:N/A (2016).
Read more (PubMed: 27537898) »
Kramer D et al. A pro-apoptotic function of iASPP by stabilizing p300 and CBP through inhibition of BRMS1 E3 ubiquitin ligase activity. Cell Death Dis6:e1634 (2015).
Read more (PubMed: 25675294) »