Overview

  • Product nameAnti-p73 antibody [EP436Y]
    See all p73 primary antibodies
  • Description
    Rabbit monoclonal [EP436Y] to p73
  • Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human p73 aa 50-150.

  • Positive control
    • WB: HeLa, Jurkat, HEK293 and NIH/3T3 cell lysates. IHC-P: Human urinary bladder carcinoma, human kidney, human liver carcinoma and mouse testis tissues. ICC/IF: HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alternative versions available:

    Anti-p73 antibody (Alexa Fluor® 647) [EP436Y] (ab197039)

    Anti-p73 antibody (HRP) [EP436Y] (ab197040)

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab40658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/500 - 1/2000. Detects a band of approximately 63 kDa (predicted molecular weight: 70 kDa).
ICC/IF 1/150 - 1/300.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Flow Cyt Use at an assay dependent concentration.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionParticipates in the apoptotic response to DNA damage. Isoforms containing the transactivation domain are pro-apoptotic, isoforms lacking the domain are anti-apoptotic and block the function of p53 and transactivating p73 isoforms. May be a tumor suppressor protein.
    • Tissue specificityExpressed in striatal neurons of patients with Huntington disease (at protein level). Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas. Highly expressed in fetal tissue.
    • Sequence similaritiesBelongs to the p53 family.
      Contains 1 SAM (sterile alpha motif) domain.
    • DomainPossesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain.
      The WW-binding motif mediates interaction with WWOX.
    • Post-translational
      modifications
      Isoform alpha (but not isoform beta) is sumoylated on Lys-627, which potentiates proteasomal degradation but does not affect transcriptional activity.
      Higher levels of phosphorylation seen in the brain from patients with Huntington disease.
      Ubiquitinated; leading to its degradation by the proteasome.
    • Cellular localizationNucleus. Accumulates in the nucleus in response to DNA damage.
    • Information by UniProt
    • Database links
    • Alternative names
      • p53 like transcription factor antibody
      • p53 related protein antibody
      • p53-like transcription factor antibody
      • p53-related protein antibody
      • p73 antibody
      • P73_HUMAN antibody
      • TP73 antibody
      • Tumor protein p73 antibody
      see all

    Anti-p73 antibody [EP436Y] images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • All lanes : Anti-p73 antibody [EP436Y] (ab40658) at 1/5000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : HEK293 whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 70 kDa
      Observed band size : 60,75 kDa (why is the actual band size different from the predicted?)
      Blocking and dilution buffer: 5% NFDM/TBST.
    • Flow Cytometry analysis of 293(human embryonic kidney) cells labeling p73 with purified ab40658 at 1/120 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    • Anti-p73 antibody [EP436Y] (ab40658) at 1/1000 dilution (purified) + NIH/3T3 whole cell lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 70 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
      Blocking and dilution buffer: 5% NFDM/TBST.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue labelling p73 with purified ab40658 at a dilution of 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling p73 with purified ab40658 at a dilution of 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/300) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human urinary bladder carcinoma tissue labelling p73 with unpurified ab40658 at a dilution of 1/100.

    • All lanes : Anti-p73 antibody [EP436Y] (ab40658) at 1/2000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 70 kDa
      Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    • Unpurified ab40658 staining p73 in murine hairy skin tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde, permeabilized using 0.1% Triton then blocked using 5% BSA for 30 minutes at 25°C. Samples were then incubated with ab40658 at a 1/100 dilution for 16 hours at 25°C. The secondary used was an Alexa Fluor® 488 conjugated goat anti-rabbit polyclonal used at a 1/1000 dilution.

      See Abreview

    References for Anti-p73 antibody [EP436Y] (ab40658)

    This product has been referenced in:
    • Venkatanarayan A  et al. IAPP-driven metabolic reprogramming induces regression of p53-deficient tumours in vivo. Nature 517:626-30 (2015). Read more (PubMed: 25409149) »
    • Zhang W  et al. ELL inhibits E2F1 transcriptional activity by enhancing E2F1 deacetylation via recruitment of histone deacetylase 1. Mol Cell Biol 34:765-75 (2014). Read more (PubMed: 24344198) »

    See all 11 Publications for this product

    Product Wall

    ab40658 was tested in IP on 293T lysate, and detected a faint smear along with two non-specific bands. As a result, we didn't recommend it for use in IP.

    The p73 antibody ab40658 should recognize all p73 isoforms, including the delta-n isoforms, except for isoform 10 according to the uniprot file below: http://www.uniprot.org/uniprot/O15350

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (hairy skin sections)
    Specification hairy skin sections
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.1% TRITON, 3'
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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    Submitted Jul 07 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"