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Product name
Anti-p75 NGF Receptor antibody
See all p75 NGF Receptor products (24) ...
Description
Goat polyclonal to p75 NGF Receptor
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Zebrafish
Immunogen
Synthetic peptide: C-AKREEVEKLLNG, corresponding to amino acids 342-353 of Human p75 NGF Receptor.
C-AKREEVEKLLNG
Positive control
WB: Human Duodenum and Human Heart lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide.
Constituents: 0.5% BSA, Tris saline. pH 7.3.
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Ab32888 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Neural Stem Cells >> Surface Molecules
Neuroscience >> Neurology process >> Growth and Development >> Neurotrophins
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Western blot - p75 NGF Receptor antibody (ab32888)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: 1/32000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 45 kDa). The observed molecular weight corresponds to earlier findings in literature (see reference provided).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
Sequence similarities
Contains 1 death domain.
Contains 4 TNFR-Cys repeats.
Domain
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1.
Post-translational
modifications
N- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
Phosphorylated on serine residues.
Cellular localization
Membrane.
Target information above from: UniProt accessionP08138
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Gp80 LNGFR antibody
- Low affinity nerve growth factor receptor antibody
- Low affinity neurotrophin receptor p75NTR antibody
see all
Database links
- Entrez Gene: 4804 Human
- Entrez Gene: 18053 Mouse
- Entrez Gene: 24596 Rat
- Entrez Gene: 323817 Zebrafish
- Entrez Gene: 368715 Zebrafish
- Omim: 162010 Human
- SwissProt: P08138 Human
- SwissProt: Q9Z0W1 Mouse
see all
Anti-p75 NGF Receptor antibody images:
Western blot - p75 NGF Receptor antibody (ab32888)
Anti-p75 NGF Receptor antibody (ab32888) at 0.3 µg/ml + Human Heart lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 45 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 hour
The observed molecular weight corresponds to earlier findings in the literature (see reference provided).
References for Anti-p75 NGF Receptor antibody (ab32888)
ab32888 has not yet been referenced specifically in any publications.
Publishing research using ab32888? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"