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An extracellular fragment from the mouse p75 receptor (amino acids 43-161).
Our Abpromise guarantee covers the use of ab8874 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Dot Blot||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||1/400. PubMed: 169737171/100.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. Ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
NG108-15 cells, a fusion of mouse neuroblastoma and rat glioma cells, were used for a FACS analysis. Cells were incubated for one hour with Ab8874 at a 1:100 dilution, and subsequently with an anti-rabbit IgG conjugated to FITC. A 96% shift was seen relative to control cells treated with secondary antibody only. (note; yellow = control, red = result).
ab11382 staining normal mouse brain tissue sections by IHC-FoFr. Sections were PFA fixed and blocked with 0.5% TNB for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 18 hours at 4°C. An Alexa Fluor® 555 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary. This image (taken at 80x) demonstrates p75 positive neurons in the basal forebrain.
This image is courtesy of an anonymous Abreview.
Image courtesy of an anonymous Abreview.
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