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p75 NGF Receptor protein (Active) (ab83678)

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Overview

  • Product namep75 NGF Receptor protein (Active)See all p75 NGF Receptor proteins and peptides ...
  • Protein descriptionRecombinant fragment corresponding to amino acids 1-250 of Human p75 NGF Receptor fused to the Fc region of Human IgG1 (aa 93-330) expressed in modified Human 293 cells. Swiss ID P08138.
  • Expression hostHEK 293 cells

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityThe ED50 of this chimera is typically 0.7 - 1.0 ug/ml as measured by its ability to neutralize beta NGF mediated proliferation of the human growth factor dependent TF-1 cell line.
  • FormLyophilised:It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
  • Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin, PBS
  • Concentration information loading...
  • Sequence notesTheoretical sequence: KEACPTGLYTHSGECCKACNLGEGVAQPC GANQTVCEPCLDSVTFSDVVSATEPCKPCTE CVGLQSM SAPCVEADDAVCRCAYGYYQDETTGRCEACRVCEAGSGLV FSCQDKQNTVC EECPDGTYSDEANHVDPCLPCTVCEDT ERQLRECTRWADAECEEIPGRWITRSTPPEGSD STAPS TQEPEAPPEQDLIASTVAGVVTTVMGSSQPVVTRGTTDNG SSNTKVDKKVEPKSC DKTHTCPPCPAPELLGGPSVFLF PPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDG VEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKC KVSNKALPAPIEKTISKAKGQ PREPQVYTLPPSRDELT KNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLD SDGS FFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYT QKSLSLSPGK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab83678 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution. This chimera migrates as a broad band between 75 and 100 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with unmodified chimera that has a predicted molecular mass of 50.8 kDa. This chimera separates into a number of isoforms with a pI between 4.0 and 6.0 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified chimera that has a predicted pI of 4.89.

    Protein info

    • Alternative names
        CD271CD271 antigenGp80 LNGFR
        Gp80-LNGFRLow affinity nerve growth factor receptorLow affinity neurotrophin receptor p75NTRLow affinity neurotrophin receptor p75NTRLow-affinity nerve growth factor receptorNerve growth factor receptorNerve growth factor receptor TNFR superfamily member 16NGF receptorNGF receptorNGFRp75 ICDp75 ICDp75 Neurotrophin receptorp75 NTRp75(NTR)p75NTRTNFR Superfamily Member 16TNFRSF16TNR16_HUMANTumor necrosis factor receptor superfamily member 16
      see all
  • FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similaritiesContains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • DomainDeath domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localizationMembrane.

    • Target information above from: UniProt accession P08138 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for p75 NGF Receptor protein (Active) (ab83678)

    ab83678 has not yet been referenced specifically in any publications.

    Publishing research using ab83678 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab83678 Submit a Question for ab83678

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    Hi,

    You have two types of p75-Fc in your catalogue

    1. http://www.abcam.com/p75-NGF-Receptor-protein-Fc-Chimera-ab83679.html
    2. http://www.abcam.com/p75-NGF-Receptor-protein-Fc-Chimera-Active-ab83678.html

    These two products d...

    Read More

    Thank you for contacting us and your interest in our products. I am sorry for the delay in getting back to you.
    Currently, only the p75 NGF Receptor protein ab83678 has been shown to have biological activity. This has been performed by measuring the ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"