Overview

  • Product nameAnti-p75 NGF Receptor antibody [2E3]
    See all p75 NGF Receptor primary antibodies
  • Description
    Rat monoclonal [2E3] to p75 NGF Receptor
  • Tested applicationsSuitable for: ICC/IF, IP, WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: mouse embryo kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab27007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
IP 1/500 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 75 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/10.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similaritiesContains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • DomainDeath domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Anti-p75 NGF Receptor antibody [2E3] images

  • Anti-p75 NGF Receptor antibody [2E3] (ab27007) + BW5147 NGFR expression cells lysate

    Secondary
    anti-rat secondary antibody conjugated to HRP

    Predicted band size : 75 kDa
    Observed band size : 75 kDa
    A chemiluminescence detection system was used.
  • Mouse embryo kidney immunostaining by ab27007.
  • Detection of cell membrane mouse Endomucin in BW5147 cell lines. Cells were stained using ab27007 and anti-rat IgG FITC conjugated mAb(blue) or the isotype control(red). Flow cytometry was performed on a FACSCaliburTM.

References for Anti-p75 NGF Receptor antibody [2E3] (ab27007)

This product has been referenced in:
  • Lopes CS  et al. CD271+ Mesenchymal Stem Cells as a Possible Infectious Niche for Leishmania infantum. PLoS One 11:e0162927 (2016). ICC/IF ; Mouse . Read more (PubMed: 27622907) »
  • Guseva D  et al. Ablation of adhesion molecule L1 in mice favours Schwann cell proliferation and functional recovery after peripheral nerve injury. Brain 132:2180-95 (2009). Read more (PubMed: 19541848) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. Our short answer is that ab27007 was raised against the whole receptor and not a portion of it, and that the region of the receptor that the antibody binds has not been mapped. Production of the antibody is described...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"