Overview

  • Product name
    Anti-p75 NGF Receptor antibody [2E3]
    See all p75 NGF Receptor primary antibodies
  • Description
    Rat monoclonal [2E3] to p75 NGF Receptor
  • Tested applications
    Suitable for: ICC/IF, IP, WB, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: mouse embryo kidney.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab27007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
IP 1/500 - 1/1000.
WB 1/500 - 1/1000. Predicted molecular weight: 75 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/10.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain
    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Images

  • Anti-p75 NGF Receptor antibody [2E3] (ab27007) + BW5147 NGFR expression cells lysate

    Secondary
    anti-rat secondary antibody conjugated to HRP

    Predicted band size : 75 kDa
    Observed band size : 75 kDa
    A chemiluminescence detection system was used.
  • Mouse embryo kidney immunostaining by ab27007.
  • Detection of cell membrane mouse Endomucin in BW5147 cell lines. Cells were stained using ab27007 and anti-rat IgG FITC conjugated mAb(blue) or the isotype control(red). Flow cytometry was performed on a FACSCaliburTM.

References

This product has been referenced in:
  • Lopes CS  et al. CD271+ Mesenchymal Stem Cells as a Possible Infectious Niche for Leishmania infantum. PLoS One 11:e0162927 (2016). ICC/IF ; Mouse . Read more (PubMed: 27622907) »
  • Garhyan J  et al. Preclinical and Clinical Evidence of Mycobacterium tuberculosis Persistence in the Hypoxic Niche of Bone Marrow Mesenchymal Stem Cells after Therapy. Am J Pathol 185:1924-34 (2015). Read more (PubMed: 26066709) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. Our short answer is that ab27007 was raised against the whole receptor and not a portion of it, and that the region of the receptor that the antibody binds has not been mapped. Production of the antibody is described...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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