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Mouse monoclonal [5C4B9] to p75 NGF Receptor
Please note that, despite having used an immunogen that is 100% homologous with the human and rat p75 NGF receptor proteins, Abcam could not get this antibody to detect p75 NGF receptor in human or rat tissue lysates. Abcam would welcome feedback from customers that have used ab86768 in either of these species!
Predicted to work with:
Synthetic peptide derived from within residues 250 - 350 of Human p75 NGF Receptor.
This antibody gave a positive signal in the following mouse tissue lysates: Brain; Spinal Cord; Heart.
This antibody clone is manufactured by Abcam.
This antibody is available as an azide-free product (see
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
firstname.lastname@example.org or you can find further information here.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS
Concentration information loading...
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 75 kDa).
Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
Contains 1 death domain.
Contains 4 TNFR-Cys repeats.
Death domain is responsible for interaction with RANBP9.
The extracellular domain is responsible for interaction with NTRK1.
N- and O-glycosylated.
O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. Phosphorylated on serine residues.
Information by UniProt
CD271 antigen antibody
Gp80 LNGFR antibody
Western blot - p75 NGF Receptor antibody [5C4B9] (ab86768)
All lanes :
Anti-p75 NGF Receptor antibody [5C4B9] (ab86768) at 5 µg/ml
Lane 1 :
Brain (Mouse) Tissue Lysate
Lane 2 :
Spinal Cord (Mouse) Tissue Lysate
Lane 3 :
Heart (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size :
Observed band size :
74 kDa (
why is the actual band size different from the predicted?
Additional bands at :
30 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"