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Anti-p75 NGF Receptor antibody images
Immunocytochemistry/ Immunofluorescence - Anti-p75 NGF Receptor antibody (ab25958)Image from Kajiya M et al., J Biol Chem, 283, 16259-76. Fig 1.; doi: 10.1074/jbc.M800668200.
Immunocytochemistry/ Immunofluorescence of HCEM cells labelling p75 NGF Receptor (green) with ab25958 at 1/500. Cells were cultured until low confluence on 35mm glass base dishes and washed with PBS. Cells were fixed with 4% paraformaldehyde in PBS and blocked by incubation with TBS containing 0.2% casein and 0.1% Triton X-100 for 20 minutes. Cells were incubated with primary antibody (1/500) overnight at 4°C. An Alexa Fluor® 488 anti-rabbit IgG (1/200) was used as the secondary antibody. Beta actin was stained with Alexa Fluor® 594 phalloidin (red).
ab25958 at 10µg/ml staining p75NGF Receptor in A20 cells by ICC/IF
References for Anti-p75 NGF Receptor antibody (ab25958)
This product has been referenced in:
Kajiya M et al. Brain-derived neurotrophic factor stimulates bone/cementum-related protein gene expression in cementoblasts. J Biol Chem283:16259-67 (2008).
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