The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/32000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 45 kDa). The observed molecular weight corresponds to earlier findings in literature (see reference provided).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
Sequence similaritiesContains 1 death domain. Contains 4 TNFR-Cys repeats.
DomainDeath domain is responsible for interaction with RANBP9. The extracellular domain is responsible for interaction with NTRK1.
Post-translational modificationsN- and O-glycosylated. O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. Phosphorylated on serine residues.
References for Anti-p75 NGF Receptor antibody (ab32888)
This product has been referenced in:
Han HW et al. The Nogo-C2/Nogo receptor complex regulates the morphogenesis of zebrafish lateral line primordium through modulating the expression of dkk1b, a Wnt signal inhibitor. PLoS One9:e86345 (2014).
Read more (PubMed: 24466042) »