Recombinant
RabMAb

Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab52987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 45 kDa).
IP 1/50.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50.
Flow Cyt 1/60.

Target

  • Function
    Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Domain
    Death domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Images

  • Immunohistochemical analysis of murine uterus tissue with adenomyosis, staining p75 NGF Receptor with ab52987.

    Antigen retrieval was performed by heat mediation in citrate buffer (pH 6). Tissue was blocked with goat serum for 15 minutes before incubating with primary antibody (1/100) overnight at 4°C. A biotinylated goat anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.
  • ICC/IF image of ab52987 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52987, 1 µg/mL) overnight at 4oC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluo® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.4 µM.

  • Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987) at 1/50000 dilution + PC12 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP labelled at 1/2000 dilution

    Predicted band size : 45 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling p75 NGF Receptor with unpurified ab52987 at 1/60 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:
  • Kojima H  et al. Enhanced cancer stem cell properties of a mitotically quiescent subpopulation of p75NTR-positive cells in esophageal squamous cell carcinoma. Int J Oncol 51:49-62 (2017). ICC/IF ; Human . Read more (PubMed: 28534989) »
  • De la Cruz-Morcillo MA  et al. p75 neurotrophin receptor and pro-BDNF promote cell survival and migration in clear cell renal cell carcinoma. Oncotarget 7:34480-97 (2016). Read more (PubMed: 27120782) »

See all 12 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Human Cell lysate - whole cell (Melanoma)
Specification
Melanoma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Abcam user community

Verified customer

Submitted May 30 2014

Vielen Dank für Ihre Email.

Gerne send ich Ihnen den Antikörper zu, doch ich bräuchte noch die Umsatzsteuernummer Ihres Institutes, damit Sie nicht unsere Britische Mehrwertsteuer bezahlen müssen...

Benutzen Sie un...

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Le pido disculpas por la larga espera, he contactado con nuestros colaboradores y mis compañeros del laboratorio para solicitar información acerca de la especificidad de los anticuerpos contra p75NTR.
De las alternativas encontradas, el único que ...

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Thanks for your reply and for the additional information. I agree it does appear that this vial of ab52987 is not working as expected. If you have purchased this antibody within the last 6 months or so I would be happy to offer a replacement, cred...

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Thank you for your enquiry. I am sorry to hear these two antibodies are giving you trouble. Just a few additional questions regarding your experiments: 1) Have you used a loading control antibody such as an antibody against beta actin to ...

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