Nerve growth factor receptor TNFR superfamily member 16 antibody
NGF receptor antibody
p75 ICD antibody
p75 Neurotrophin receptor antibody
p75 NTR antibody
TNFR Superfamily Member 16 antibody
Tumor necrosis factor receptor superfamily member 16 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)Image from Li Y et al., Reprod Biol Endocrinol. 2011 Mar 8;9:30. Fig 2.; doi:10.1186/1477-7827-9-30; 8 March 2011, Reproductive Biology and Endocrinology 2011, 9:30
Immunohistochemical analysis of murine uterus tissue with adenomyosis, staining p75 NGF Receptor with ab52987.
Antigen retrieval was performed by heat mediation in citrate buffer (pH 6). Tissue was blocked with goat serum for 15 minutes before incubating with primary antibody (1/100) overnight at 4°C. A biotinylated goat anti-rabbit IgG was used as the secondary antibody and staining was detected using DAB.
ICC/IF image of ab52987 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52987, 1 µg/mL) overnight at 4oC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluo® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.4 µM.
Western blot - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)
Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987) at 1/50000 dilution + PC12 cell lysate at 10 µg
Secondary goat anti-rabbit HRP labelled at 1/2000 dilution
Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling p75 NGF Receptor with unpurified ab52987 at 1/60 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
Other - Anti-p75 NGF Receptor antibody [EP1039Y] (ab52987)
Equilibrium disassociation constant (KD) Learn more about KD
Kojima H et al. Enhanced cancer stem cell properties of a mitotically quiescent subpopulation of p75NTR-positive cells in esophageal squamous cell carcinoma. Int J Oncol51:49-62 (2017).
Read more (PubMed: 28534989) »
De la Cruz-Morcillo MA et al. p75 neurotrophin receptor and pro-BDNF promote cell survival and migration in clear cell renal cell carcinoma. Oncotarget7:34480-97 (2016).
Read more (PubMed: 27120782) »