Anti-p75 NGF Receptor antibody [MC-192] (ab6172)

Overview

  • Product nameAnti-p75 NGF Receptor antibody [MC-192]
    See all p75 NGF Receptor primary antibodies
  • Description
    Mouse monoclonal [MC-192] to p75 NGF Receptor
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Rat PC-12 cells.

  • Positive control
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.
  • General notes


    A well characterised and versatile monoclonal antibody, clone number MC-192, that is of central importance in the study of LANGF (p75-NGF) receptor.

Properties

Applications

Our Abpromise guarantee covers the use of ab6172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20213527

Target

  • FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
  • Sequence similaritiesContains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • DomainDeath domain is responsible for interaction with RANBP9.
    The extracellular domain is responsible for interaction with NTRK1.
  • Post-translational
    modifications
    N- and O-glycosylated.
    O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
    Phosphorylated on serine residues.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD271 antibody
    • CD271 antigen antibody
    • Gp80 LNGFR antibody
    • Gp80-LNGFR antibody
    • Low affinity nerve growth factor receptor antibody
    • Low affinity neurotrophin receptor p75NTR antibody
    • Low-affinity nerve growth factor receptor antibody
    • Nerve growth factor receptor antibody
    • Nerve growth factor receptor TNFR superfamily member 16 antibody
    • NGF receptor antibody
    • Ngfr antibody
    • p75 ICD antibody
    • p75 Neurotrophin receptor antibody
    • p75 NTR antibody
    • p75(NTR) antibody
    • p75NTR antibody
    • TNFR Superfamily Member 16 antibody
    • TNFRSF16 antibody
    • TNR16_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 16 antibody
    see all

Anti-p75 NGF Receptor antibody [MC-192] images

  • ab6172 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6172 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-p75 NGF Receptor antibody [MC-192] (ab6172)

This product has been referenced in:
  • Nelson AR  et al. Noradrenergic sympathetic sprouting and cholinergic reinnervation maintains non-amyloidogenic processing of AßPP. J Alzheimers Dis 38:867-79 (2014). IHC ; Rat . Read more (PubMed: 24081376) »
  • Barres BA Designing and troubleshooting immunopanning protocols for purifying neural cells. Cold Spring Harb Protoc 2014:1342-7 (2014). Read more (PubMed: 25447277) »

See all 5 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Cat Tissue sections (brain stem)
Specification brain stem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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Submitted Sep 12 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Rat Tissue sections (brainstem slice)
Specification brainstem slice
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 12 2014

Thank you very much for your interest in ab6172.

To our knowledge, ab6172 has not been tested in human. Therefore, I can offer a discount off a future purchase if you buy ab6172 now, test it inhuman and submit feedback to us in the form of a...

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Thank you for your enquiry. I apologise for any confusion that has been caused with product Ab6172. There is a concentration value cited on the product data sheet, as 1.0mg/ml. You should have received a copy of this data sheet upon receipt of the prod...

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Dear Dr Hilker I am afraid that this antibody only recognises rat Low Affinity Nerve Growth Factor receptor. Regards Michael

Thank you for your enquiry. This antibody is not currently available biotinylated, however we do offer a conjugation service for £150 pounds sterling or $225 US dollars. We would need to biotinylate at least 1mg antibody; the price listed on our web si...

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Yes, this antibody is ideal for FACS of PC12 cells.

Yes, the antibody is excellent in these applications.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"