Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

Overview

  • Product nameAnti-p75 NGF Receptor antibody [NGFR5]
    See all p75 NGF Receptor primary antibodies
  • Description
    Mouse monoclonal [NGFR5] to p75 NGF Receptor
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human, Ferret, Monkey
    Predicted to work with: Rabbit, Cat, BaboonDoes not react with: Mouse, Rat
  • Immunogen

    NGFR from A875 melanoma cells.

  • EpitopeAmino acids 1 - 160.
  • Positive control
    • Melanoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab3125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for WB.
  • Target

    • FunctionLow affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
    • Sequence similaritiesContains 1 death domain.
      Contains 4 TNFR-Cys repeats.
    • DomainDeath domain is responsible for interaction with RANBP9.
      The extracellular domain is responsible for interaction with NTRK1.
    • Post-translational
      modifications
      N- and O-glycosylated.
      O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc.
      Phosphorylated on serine residues.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD271 antibody
      • CD271 antigen antibody
      • Gp80 LNGFR antibody
      • Gp80-LNGFR antibody
      • Low affinity nerve growth factor receptor antibody
      • Low affinity neurotrophin receptor p75NTR antibody
      • Low-affinity nerve growth factor receptor antibody
      • Nerve growth factor receptor antibody
      • Nerve growth factor receptor TNFR superfamily member 16 antibody
      • NGF receptor antibody
      • Ngfr antibody
      • p75 ICD antibody
      • p75 Neurotrophin receptor antibody
      • p75 NTR antibody
      • p75(NTR) antibody
      • p75NTR antibody
      • TNFR Superfamily Member 16 antibody
      • TNFRSF16 antibody
      • TNR16_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 16 antibody
      see all

    Anti-p75 NGF Receptor antibody [NGFR5] images

    • ICC/IF image of ab3125 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3125, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • ab3125 staining p75 NGF Receptor in Human cholinergic neurons by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formalin and subjected to heat-mediated antigen retrieval in sodium citrate buffer prior to blocking with 2.5% serum for 1 hour 30 minutes at 21°C. The primary antibody was diluted 1/250 in normal horse serum blocking buffer and incubated with the sample for 65 hours at 4°C.

      See Abreview

    • ab3125 staining p75 NGF Receptor in human melanoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using Tris EDTA pH 9.0. Samples were then permeabilized using 0.25% Triton X-100, blocked for 1 hour at room temperature and then incubated with ab3125 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 594 (red) conjugated donkey anti-mouse polyclonal used at a 1/100 dilution. (Green) tumor cells, (blue) DAPI.

      See Abreview

    References for Anti-p75 NGF Receptor antibody [NGFR5] (ab3125)

    This product has been referenced in:
    • Twenhafel NA  et al. Pathology of experimental aerosol zaire ebolavirus infection in rhesus macaques. Vet Pathol 50:514-29 (2013). Read more (PubMed: 23262834) »
    • Cuthbert RJ  et al. Induced periosteum a complex cellular scaffold for the treatment of large bone defects. Bone 57:484-92 (2013). IHC-P, Flow Cyt ; Human . Read more (PubMed: 23954755) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Bone Marrow)
    Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
    Permeabilization No
    Specification Bone Marrow
    Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
    Fixative 10% NBF, 10% Formic Acid Decalcification
    Username

    Dr. Faye Doherty

    Verified customer

    Submitted Mar 29 2016

    We do not have any free samples or small samples of any of our antibodies but both ab12228 and ab3125 will be covered by our guarantee for one year if you plan on using them for flow cytometry with samples that are one of the species listed on the data...

    Read More

    Merci pour votre émail. Je suis désolée que nous pouvons par regarder les images du coup. Nous sommes en Angleterre, et je pense que ça va devenir compliqué pour envoyer les lames.

    Je vais chercher pour trou...

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    Thank you for your inquiry.

    I am pleased to let you know that we currently have a number of CD271 antibodies (24 in total); we sell them under the alternative name 'p75-NGF receptor', such as the rabbit polyclonal antibody ab8874 and the mou...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Melanoma)
    Specification Melanoma
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris EDTA pH9.0
    Permeabilization Yes - 0.25%Triton X-100
    Blocking step DakoCytomation X0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Username

    Hongwei Shao

    Verified customer

    Submitted Nov 09 2011

    Merci pour ces précisions. N'hésitez pas à nous contacter de nouveau si vous rencontrez des difficultés.

    Le tissu cérébral humain est en effet un tissu de choix pour l'expression de TrkA, NGF et le récepteur p75 NGF. Avez-vous reçu ces 3 anticorps en même temps (même colis de livraison)?

    Merci de nous avoir contactés. Je suis désolé d'apprendre que vous avez eu des difficultés à obtenir de bons résultats avec ab3125, ab6199, ab37837 en IHC-P. Notre politique est d'offrir tout d'abord la meilleure assistance technique. S...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Cholinergic neurons, NBM)
    Specification Cholinergic neurons, NBM
    Fixative formalin
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2.5% · Temperature: 21°C
    Username

    Ms. Amy Nelson

    Verified customer

    Submitted May 28 2011

    Thank you for your enquiry. We do not have MSDSs specific for each antiserum. The material safety datasheets are issued based on the inclusion of additional components such as Na Azide and Glycerol in addition to specific componds that we sell for ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"