Overview

  • Product nameAnti-p90 Autoantigen antibody
    See all p90 Autoantigen primary antibodies
  • Description
    Rabbit polyclonal to p90 Autoantigen
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within the amino acids 550-600 of Human p90 Autoantigen, NP_065941.1

  • Positive control
    • Human colon carcinoma. Human ovarian carcinoma.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionOncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
  • Tissue specificityExpressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
  • Cellular localizationMembrane. Cytoplasm. Slightly concentrates in the perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cancerous inhibitor of PP2A antibody
    • Cancerous Inhibitor of Protein Phosphatase 2A antibody
    • CIP2A antibody
    • CIP2A_HUMAN antibody
    • FLJ12850 antibody
    • Kiaa1524 antibody
    • MGC163436 antibody
    • p90 antibody
    • p90 autoantigen antibody
    • Protein CIP2A antibody
    see all

Anti-p90 Autoantigen antibody images

  • Detection of Human p90 Autoantigen by Immunohistochemistry.
    Sample: FFPE section of Human colon carcinoma.
    Antibody: ab84547 used at a dilution of 1/250.
    Detection: DAB staining.
  • Detection of Human p90 Autoantigen by Immunohistochemistry.
    Sample: FFPE section of Human ovarian carcinoma.
    Antibody: ab84547 used at a dilution of 1/250.
    Detection: DAB staining.
  • Immunohistochemical analysis of Human breast tumour tissue, staining p90 Autoantigen with ab84547 at 1/100 dilution.

References for Anti-p90 Autoantigen antibody (ab84547)

This product has been referenced in:
  • Liu CY  et al. Tamoxifen induces apoptosis through cancerous inhibitor of protein phosphatase 2A dependent phospho-Akt inactivation in estrogen-receptor negative human breast cancer cells. Breast Cancer Res 16:431 (2014). IHC-P . Read more (PubMed: 25228280) »
  • Yu HC  et al. Erlotinib derivative inhibits hepatocellular carcinoma by targeting CIP2A to reactivate protein phosphatase 2A. Cell Death Dis 5:e1359 (2014). IHC-P . Read more (PubMed: 25077545) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: RT°C
Antigen retrieval step None
Sample Human Tissue sections (Breast)
Specification Breast
Permeabilization No
Fixative Formaldehyde
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Submitted Sep 25 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"