Overview

  • Product nameAnti-p90 Autoantigen antibody
    See all p90 Autoantigen primary antibodies
  • Description
    Rabbit polyclonal to p90 Autoantigen
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within the amino acids 550-600 of Human p90 Autoantigen, NP_065941.1

  • Positive control
    • Human colon carcinoma. Human ovarian carcinoma.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionOncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
    • Tissue specificityExpressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
    • Cellular localizationMembrane. Cytoplasm. Slightly concentrates in the perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancerous inhibitor of PP2A antibody
      • Cancerous Inhibitor of Protein Phosphatase 2A antibody
      • CIP2A antibody
      • CIP2A_HUMAN antibody
      • FLJ12850 antibody
      • Kiaa1524 antibody
      • MGC163436 antibody
      • p90 antibody
      • p90 autoantigen antibody
      • Protein CIP2A antibody
      see all

    Anti-p90 Autoantigen antibody images

    • Detection of Human p90 Autoantigen by Immunohistochemistry.
      Sample: FFPE section of Human colon carcinoma.
      Antibody: ab84547 used at a dilution of 1/250.
      Detection: DAB staining.
    • Detection of Human p90 Autoantigen by Immunohistochemistry.
      Sample: FFPE section of Human ovarian carcinoma.
      Antibody: ab84547 used at a dilution of 1/250.
      Detection: DAB staining.
    • Immunohistochemical analysis of Human breast tumour tissue, staining p90 Autoantigen with ab84547 at 1/100 dilution.

    References for Anti-p90 Autoantigen antibody (ab84547)

    This product has been referenced in:
    • Liu CY  et al. Tamoxifen induces apoptosis through cancerous inhibitor of protein phosphatase 2A dependent phospho-Akt inactivation in estrogen-receptor negative human breast cancer cells. Breast Cancer Res 16:431 (2014). IHC-P . Read more (PubMed: 25228280) »
    • Tseng LM  et al. CIP2A is a target of bortezomib in human triple negative breast cancer cells. Breast Cancer Res 14:R68 (2012). IHC-P ; Human . Read more (PubMed: 22537901) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: RT°C
    Antigen retrieval step None
    Sample Human Tissue sections (Breast)
    Specification Breast
    Permeabilization No
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 25 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"