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Rabbit polyclonal to p90 Autoantigen
Synthetic peptide, corresponding to a region between amino acids 550-600 of Human p90 Autoantigen (NP_065941.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8
Concentration information loading...
Immunogen affinity purified
ab99396 was affinity purified using an epitope specific to p90 Autoantigen immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
Information by UniProt
Cancerous inhibitor of PP2A antibody
Cancerous Inhibitor of Protein Phosphatase 2A antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p90 Autoantigen antibody (ab99396)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling p90 Autoantigen with ab99396 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation - Anti-p90 Autoantigen antibody (ab99396)
Detection of p90 Autoantigen by Western Blot of Immunprecipitate.
anti-p90 Autoantigen at 0.1µg/ml staining p90 Autoantigen in HeLa whole cell lysate immunoprecipitated using ab99396 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"