Overview

  • Product name
    Anti-p90 Autoantigen antibody
    See all p90 Autoantigen primary antibodies
  • Description
    Rabbit polyclonal to p90 Autoantigen
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 550-600 of Human p90 Autoantigen (NP_065941.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab99396 was affinity purified using an epitope specific to p90 Autoantigen immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
    • Tissue specificity
      Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
    • Cellular localization
      Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cancerous inhibitor of PP2A antibody
      • Cancerous Inhibitor of Protein Phosphatase 2A antibody
      • CIP2A antibody
      • CIP2A_HUMAN antibody
      • FLJ12850 antibody
      • Kiaa1524 antibody
      • MGC163436 antibody
      • p90 antibody
      • p90 autoantigen antibody
      • Protein CIP2A antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling p90 Autoantigen with ab99396 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of p90 Autoantigen by Western Blot of Immunprecipitate.
      anti-p90 Autoantigen at 0.1µg/ml staining p90 Autoantigen in HeLa whole cell lysate immunoprecipitated using ab99396 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
      Detection: Chemiluminescence with exposure time of 3 seconds.

    References

    ab99396 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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