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Rabbit polyclonal to p90 Autoantigen
Mouse, Rat, Human
Predicted to work with:
Cow, Chimpanzee, Rhesus monkey, Orangutan, Bat, Elephant
Synthetic peptide, corresponding to a region within amino acids 853-903 of Human p90 Autoantigen (NP_065941.1).
HeLa and 293T cell lines
This antibody gave a positive result when used in the following formaldehyde fixed cell lines: MDAMB231
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
Use at 2-5 µg/mg of lysate.
Use a concentration of 1 µg/ml.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
Information by UniProt
Cancerous inhibitor of PP2A antibody
Cancerous Inhibitor of Protein Phosphatase 2A antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p90 Autoantigen antibody (ab99518)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling p90 Autoantigen with ab99518 at 1/200 (1µg/ml). Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-p90 Autoantigen antibody (ab99518)
ab99518 stained MDAMB231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99518 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (
ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-p90 Autoantigen antibody (ab99518)
All lanes : Anti-p90 Autoantigen antibody (ab99518) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique. Predicted band size: 102 kDa Exposure time: 3 seconds
Immunoprecipitation - Anti-p90 Autoantigen antibody (ab99518)
1 mg of HeLa cell lysate immunoprecipitated with ab99518 at 3 µg/mg lysate; 20% of immunoprecipitate loaded
Western Blot: p90 Autoantigen antibody (ab99518) at 0.1 µg/ml
developed using the ECL technique
Exposure time : 3 seconds Predicted band size : 102 kDa
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