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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 102 kDa.
Use at 2-5 µg/mg of lysate.
Use a concentration of 1 µg/ml.
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function Oncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
Tissue specificity Expressed at low levels in most of the tissues. Overexpressed in head-and-neck squamous cell carcinomas (HNSCC). Present in liver cancer cells (at protein level).
Cellular localization Membrane. Cytoplasm. Slightly concentrates in the perinuclear region.
Information by UniProt
Cancerous inhibitor of PP2A antibody
Cancerous Inhibitor of Protein Phosphatase 2A antibody
Anti-p90 Autoantigen antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p90 Autoantigen antibody (ab99518)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling p90 Autoantigen with ab99518 at 1/200 (1µg/ml). Detection: DAB.
Immunocytochemistry/ Immunofluorescence - Anti-p90 Autoantigen antibody (ab99518)
ab99518 stained MDAMB231 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab99518 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (
ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - p90 Autoantigen antibody (ab99518)
All lanes : Anti-p90 Autoantigen antibody (ab99518) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 102 kDa Exposure time : 3 seconds
Immunoprecipitation - p90 Autoantigen antibody (ab99518)
Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99518 at 3 µg/mg lysate; 20% of immunoprecipitate loaded
Western Blot: p90 Autoantigen antibody (ab99518) at 0.1 µg/ml
developed using the ECL technique
Exposure time : 3 seconds Predicted band size : 102 kDa
References for Anti-p90 Autoantigen antibody (ab99518)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"