Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining
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I purchased ab7860 (anti-NBS1) on Fred Hutchinson Cancer Research Center PO# 563112. I was expecting to receive 100 ug of antibody but received less then 75 ug. I had planed a series of experiments that were dependent upon ~ 90 ug of Ab. Could you send me another 25 ug of Ab? Tube from GeneTex labeled: 100 mg [sic] (1 mg/ml)
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ANSWER: |
I apologize for the shortage of ab7860 that you received and I'm sending you a replacement vial free of charge. For your record, it's order number 36199 and is being sent to your attention/free replacement. It will be shipping Monday and you should receive it Tuesday. Please let me know if there are any problems. |
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Has this antibody been tested for use in immunofluorescence? |
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ANSWER: |
Yes, it has been used for IF using transfected tissue culture cells expressing ER-beta. There is no cross reactivity with ER-alpha in these experiments. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-p95 NBS1 antibody [1D7] (ab7860) at 1/500 dilution
Lane 1 : HeLa whole cell extract
Lane 2 : HepG2 whole cell extract
Lysates/proteins at 40 µg per lane.
Predicted band size : 91 kDa
Observed band size : 97 kDa (why is the actual band size different from the predicted?)
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