Anti-p95 NBS1 antibody [Y112] - ChIP Grade (ab32074)

what is the concentration of Anti-p95 NBS1 antibody [Y112] - ChIP Grade (ab32074) lot GR4228-5?

ANSWER:

The concentration of ab32074 lot GR4228-5is : 0.645 mg/mL.

Batch variability in ChIP. Would like to replace vials from alot that did not work well in ChIPwith vials from adifferent lot.

ANSWER:

Thank you for your call today and for keeping me updated about this situation.

I am sending the three vials thatwe havein stock. These vials will be arriving on the free of charge order 1060035, and I expect them to arrive tomorrow.

I've also issued credit note***for one vial of ab32074. The full purchase price will be refunded back to your credit card pending approval from our accounting department. If you have any questions about this credit note, please contact mailto:us.credits@abcam.com.

I apologize again for the batch variability of this antibody. Please let me know if you have any questions or if there is anything else that we can do for you.

Dear Madam / Sir,

A user needs a information about positive control tissue p95 NBS1 antibody [Y112] (ab32074). It is stated that Human skin carcinoma as positive control tissue on the datasheet. But It isn't clear which types of skin cancer. Could you please clear that?

Thank you.

Best regards,

ANSWER:

Thank you for your enquiry.

I can confirm that p95 NBS1 antibody [Y112] (ab32074) was tested onsquamous cell carcinoma of the skin. This would be a suitable positive control.

I hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

What is the concentration of this antibody, lot #GR50908-2?

ANSWER:

Thank you for contacting Abcam.



The concentration for ab32074, lot #GR50908-2 is *****mg/mL.



Please let me know if there is anything else I can help you with.

Lot GR4228-7 showed enrichment for negative control gene with mouse lymphocyte samples. Is lot GR50908-2 better for ChIP?

ANSWER:

Thanks for your call today and for letting us know about the trouble with ab32074 in ChIP.

As we discussed, I am sending a free of charge vial of ab32074 from lot GR50908-2 on the order ***, which should arrive tomorrow.

I've looked through our records, and we do not test this antibody in-house in ChIP. This application was added to our datasheet based on results in the following article-

http://www.ncbi.nlm.nih.gov/pubmed/21255732?dopt=Abstract

So unfortunately I don't have any data for each of these lots in ChIP, but I am hopeful that the new vial will work better for you. Please keep me updated about the results with this vial, and let me know if you have any questions or need anything else from me.