p95 NBS1 antibody [Y112] (ab32074)

Product Name 

p95 NBS1 antibody [Y112]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [Y112] to p95 NBS1

Immunogen 

Synthetic peptide corresponding to residues near C terminus of human p95 NBS1.

Reacts with 

Hu, Ms

Does not react with 
(species key)

Rat

Specificity 

ab32074 recognises p95 NBS1.

Tested applications 
(see key)

ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: 1/50.
IHC-P: 1/50.
IP: 1/60.
WB: 1/500. Predicted molecular weight: 85 kDa.

Is unsuitable for FACS.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human skin carcinoma, HeLa cell lysate.

Cellular localization 

Nuclear

Research areas 

Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Homologous Recomb.
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> Non Homol. End Joining

Relevance 

The p95 gene (identical to NBS1 and nibrin) is a member of the hMre11/hRad50 double-strand break complex (MRN complex). This protein complex has been implicated in Nijmegen breakage syndrome, an autosomal recessive disorder marked by increased cancer incidence, cell cycle checkpoint deficits, and ionizing radiation sensitivity, thus revealing a direct molecular link between double-strand break repair and cell cycle checkpoint functions. In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells. NBS1 is expressed ubiquitously and presents high levels in testis.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

Y112

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 50mM Tris glycine, 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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