Overview

  • Product nameAnti-p95 NBS1 antibody
    See all p95 NBS1 primary antibodies
  • Description
    Rabbit polyclonal to p95 NBS1
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide (Human) corresponding to a region between residue 704 and 754 of human p95 NBS1 (NP_002476.2).

  • Positive control
    • Human Anaplastic Thyroid Carcinoma, Breast Carcinoma, Ovarian Carcinoma, Prostate Carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab84488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Likely to work with frozen sections.
ICC/IF 1/200.

Target

  • FunctionComponent of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
  • Tissue specificityUbiquitous. Expressed at high levels in testis.
  • Involvement in diseaseNijmegen breakage syndrome
    Breast cancer
    Aplastic anemia
    Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
  • Sequence similaritiesContains 1 BRCT domain.
    Contains 1 FHA domain.
  • DomainThe FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
    The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
    The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
  • Post-translational
    modifications
    Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
  • Cellular localizationNucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT V1 antibody
    • AT V2 antibody
    • ATV antibody
    • Cell cycle regulatory protein p95 antibody
    • FLJ10155 antibody
    • MGC87362 antibody
    • Nbn antibody
    • NBN_HUMAN antibody
    • NBS 1 antibody
    • NBS antibody
    • NBS1 antibody
    • Nibrin antibody
    • Nijmegen breakage syndrome 1 (nibrin) antibody
    • Nijmegen breakage syndrome antibody
    • Nijmegen breakage syndrome protein 1 antibody
    • p95 antibody
    • p95 protein of the MRE11/RAD50 complex antibody
    see all

Anti-p95 NBS1 antibody images

  • IHC analysis of FFPE section of human breast carcinoma using ab84488 at a dilution of 1/250. Detection: DAB staining.
  • IHC analysis of FFPE section of human breast carcinoma using ab84488 at a dilution of 1/250. Detection: DAB staining.
  • ab84488 (1/200) staining p95 NBS1 in assynchronous Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X-100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.

    See Abreview

References for Anti-p95 NBS1 antibody (ab84488)

ab84488 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Dec 13 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"