Anti-p95 NBS1 (phospho S278) antibody (ab111373)


  • Product nameAnti-p95 NBS1 (phospho S278) antibody
    See all p95 NBS1 primary antibodies
  • Description
    Rabbit polyclonal to p95 NBS1 (phospho S278)
  • Specificityab111373 detects endogenous levels of p95 NBS1 only when phosphorylated at serine 278.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from Human p95 NBS1 around the phosphorylation site of serine 278 (T-N-SP-Q-T).

  • Positive control
    • HUVEC cell extracts, treated with foskolin; NIH3T3 cells.



Our Abpromise guarantee covers the use of ab111373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 84 kDa.
ICC/IF 1/100 - 1/500.


  • FunctionComponent of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
  • Tissue specificityUbiquitous. Expressed at high levels in testis.
  • Involvement in diseaseNijmegen breakage syndrome
    Breast cancer
    Aplastic anemia
    Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
  • Sequence similaritiesContains 1 BRCT domain.
    Contains 1 FHA domain.
  • DomainThe FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
    The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
    The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
  • Post-translational
    Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
  • Cellular localizationNucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT V1 antibody
    • AT V2 antibody
    • ATV antibody
    • Cell cycle regulatory protein p95 antibody
    • FLJ10155 antibody
    • MGC87362 antibody
    • Nbn antibody
    • NBN_HUMAN antibody
    • NBS 1 antibody
    • NBS antibody
    • NBS1 antibody
    • Nibrin antibody
    • Nijmegen breakage syndrome 1 (nibrin) antibody
    • Nijmegen breakage syndrome antibody
    • Nijmegen breakage syndrome protein 1 antibody
    • p95 antibody
    • p95 protein of the MRE11/RAD50 complex antibody
    see all

Anti-p95 NBS1 (phospho S278) antibody images

  • All lanes : Anti-p95 NBS1 (phospho S278) antibody (ab111373) at 1/500 dilution

    Lane 1 : HUVEC cell extracts, treated with Forskolin (40nM, 30mins)
    Lane 2 : HUVEC cell extracts, treated with Forskolin (40nM, 30mins) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 84 kDa
  • ab111373 at 1/100 dilution staining p95 NBS1 in NIH3T3 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.

References for Anti-p95 NBS1 (phospho S278) antibody (ab111373)

ab111373 has not yet been referenced specifically in any publications.

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