Overview

  • Product name
    Anti-p95 NBS1 (phospho S343) antibody
    See all p95 NBS1 primary antibodies
  • Description
    Rabbit polyclonal to p95 NBS1 (phospho S343)
  • Specificity
    Detects endogenous levels of p95 NBS1 only when phosphorylated at Serine 343.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized phosphopeptide derived from human p95/NBS1 around the phosphorylation site of serine 343 (S-L-SP-Q-G).

  • Positive control
    • Jurkat cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab47272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 118 kDa (predicted molecular weight: 85 kDa).
ELISA 1/4000.

Target

  • Function
    Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
  • Tissue specificity
    Ubiquitous. Expressed at high levels in testis.
  • Involvement in disease
    Nijmegen breakage syndrome
    Breast cancer
    Aplastic anemia
    Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
  • Sequence similarities
    Contains 1 BRCT domain.
    Contains 1 FHA domain.
  • Domain
    The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
    The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
    The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
  • Post-translational
    modifications
    Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
  • Cellular localization
    Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
  • Information by UniProt
  • Database links
  • Alternative names
    • AT V1 antibody
    • AT V2 antibody
    • ATV antibody
    • Cell cycle regulatory protein p95 antibody
    • FLJ10155 antibody
    • MGC87362 antibody
    • Nbn antibody
    • NBN_HUMAN antibody
    • NBS 1 antibody
    • NBS antibody
    • NBS1 antibody
    • Nibrin antibody
    • Nijmegen breakage syndrome 1 (nibrin) antibody
    • Nijmegen breakage syndrome antibody
    • Nijmegen breakage syndrome protein 1 antibody
    • p95 antibody
    • p95 protein of the MRE11/RAD50 complex antibody
    see all

Anti-p95 NBS1 (phospho S343) antibody images

  • All lanes : Anti-p95 NBS1 (phospho S343) antibody (ab47272) at 1/500 dilution

    Lane 1 : 5-30ug Jurkat cell extract, treated with immunogenic peptide.
    Lane 2 : 5-30ug Jurkat cell extract, untreated.

    Secondary
    Alkaline Phosphatase Goat Anti IgG (H+L)

    Predicted band size : 85 kDa
  • All lanes : Anti-p95 NBS1 (phospho S343) antibody (ab47272) at 1/500 dilution

    Lane 1 : Unirradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate
    Lane 2 : Irradiated Human lymphoblastoid cell lines from normal individual - Whole cell lysate
    Lane 3 : Unirradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate
    Lane 4 : Irradiated Human lymphoblastoid cell lines from classical A-T patient (no ATM kinase expressed) - Whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    An HRP-conjugated Goat polyclonal to rabbit IgG at 1/3000 dilution

    Predicted band size : 85 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-p95 NBS1 (phospho S343) antibody (ab47272)

This product has been referenced in:

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Epithelial Lung Fibroblasts)
Loading amount
15 µg
Specification
MRC5VA Epithelial Lung Fibroblasts
Treatment
30GY Ionising Radiation
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Apr 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblastoid cell lines)
Loading amount
50 µg
Specification
lymphoblastoid cell lines
Treatment
30 mins after exposure to 5Gy IR
Gel Running Conditions
Reduced Denaturing (6% Tris/Bicine gel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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