Anti-p95 NBS1 (phospho S343) antibody [EP178] (ab109453)

Overview

  • Product nameAnti-p95 NBS1 (phospho S343) antibody [EP178]
    See all p95 NBS1 primary antibodies
  • Description
    Rabbit monoclonal [EP178] to p95 NBS1 (phospho S343)
  • Specificityab109453 only detects p95 NBS1 phosphorylated on Serine 343.
  • Tested applicationsSuitable for: WB, IP, ICCmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide. within Human p95 NBS1 (phospho S343). The exact sequence is proprietary.
    Database link: O60934

  • Positive control
    • WB: Jurkat, HeLa cell lysates (treated and untreated with Etopside).
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 84 kDa).
IP 1/50.
ICC 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IHC-P.
  • Target

    • FunctionComponent of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
    • Tissue specificityUbiquitous. Expressed at high levels in testis.
    • Involvement in diseaseNijmegen breakage syndrome
      Breast cancer
      Aplastic anemia
      Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
    • Sequence similaritiesContains 1 BRCT domain.
      Contains 1 FHA domain.
    • DomainThe FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
      The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
      The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
    • Post-translational
      modifications
      Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
    • Cellular localizationNucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
    • Information by UniProt
    • Database links
    • Alternative names
      • AT V1 antibody
      • AT V2 antibody
      • ATV antibody
      • Cell cycle regulatory protein p95 antibody
      • FLJ10155 antibody
      • MGC87362 antibody
      • Nbn antibody
      • NBN_HUMAN antibody
      • NBS 1 antibody
      • NBS antibody
      • NBS1 antibody
      • Nibrin antibody
      • Nijmegen breakage syndrome 1 (nibrin) antibody
      • Nijmegen breakage syndrome antibody
      • Nijmegen breakage syndrome protein 1 antibody
      • p95 antibody
      • p95 protein of the MRE11/RAD50 complex antibody
      see all

    Anti-p95 NBS1 (phospho S343) antibody [EP178] images

    • All lanes : Anti-p95 NBS1 (phospho S343) antibody [EP178] (ab109453) at 1/5000 dilution

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma) cells whole cell lysates
      Lane 2 : HeLa (human cervix adenocarcinoma) cells were treated with Etopside whole cell lysates
      Lane 3 : HeLa (human cervix adenocarcinoma) cells were treated with Etopside whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 84 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      Blocking and diluting buffer: 5% NFDM/TBST

    • Dot blot analysis of p95 NBS1 (pS343) peptide (Lane 1) and p95 NBS1 non-phospho peptide (Lane 2) labelling p95 NBS1 (phospho S343) with ab109453 at a dilution of 1/1000. A Peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    References for Anti-p95 NBS1 (phospho S343) antibody [EP178] (ab109453)

    ab109453 has not yet been referenced specifically in any publications.

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