Anti-p95 NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)

Overview

• Product name
  Anti-p95 NBS1 (phospho S432) antibody [EPR2470Y]
  See all p95 NBS1 primary antibodies
• Description
  Rabbit monoclonal [EPR2470Y] to p95 NBS1 (phospho S432)
• Specificity
  ab75778 detects p95 NBS1 that is phosphorylated on serine 432.
• Tested applications
  Suitable for: IHC-Pmore details
  Unsuitable for: Flow Cyt,ICC,IP or WB
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A phospho-specific peptide corresponding to residues surrounding serine 432 of human p95 NBS1

• Positive control
  • HeLa cell lysates, untreated or treated with doxorubincin and human testis tissue.
• General notes

  This product is a recombinant rabbit monoclonal antibody.

  We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

  Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
• Concentration information loading...
• Purity
  Protein A purified
• Clonality
  Monoclonal
• Clone number
  EPR2470Y
• Isotype
  IgG
• Research areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Non Homol. End Joining

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Damage & Repair
  • Homologous Recomb.

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Other

Applications

Target

• Function
  Component of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
• Tissue specificity
  Ubiquitous. Expressed at high levels in testis.
• Involvement in disease
  Nijmegen breakage syndrome
  Breast cancer
  Aplastic anemia
  Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
• Sequence similarities
  Contains 1 BRCT domain.
  Contains 1 FHA domain.
• Domain
  The FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
  The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
  The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
• Post-translational
  modifications
  Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
• Cellular localization
  Nucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.

• Target information above from: UniProt accession O60934 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 4683 Human
  • Omim: 602667 Human
  • SwissProt: O60934 Human
  • Unigene: 492208 Human
• Alternative names
  • AT V1 antibody
  • AT V2 antibody
  • ATV antibody

  • Cell cycle regulatory protein p95 antibody
  • FLJ10155 antibody
  • MGC87362 antibody
  • Nbn antibody
  • NBN_HUMAN antibody
  • NBS 1 antibody
  • NBS antibody
  • NBS1 antibody
  • Nibrin antibody
  • Nijmegen breakage syndrome 1 (nibrin) antibody
  • Nijmegen breakage syndrome antibody
  • Nijmegen breakage syndrome protein 1 antibody
  • p95 antibody
  • p95 protein of the MRE11/RAD50 complex antibody

  see all

Protocols

• Immunohistochemistry protocols