Anti-p95 NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)

Overview

  • Product nameAnti-p95 NBS1 (phospho S432) antibody [EPR2470Y]
    See all p95 NBS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR2470Y] to p95 NBS1 (phospho S432)
  • Specificityab75778 detects p95 NBS1 that is phosphorylated on serine 432.
  • Tested applicationsSuitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC,IP or WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho-specific peptide corresponding to residues surrounding serine 432 of human p95 NBS1

  • Positive control
    • HeLa cell lysates, untreated or treated with doxorubincin and human testis tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab75778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/150 - 1/300. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol.

For unpurified use at 1/100 - 1/250.

  • Application notesIs unsuitable for Flow Cyt,ICC,IP or WB.
  • Target

    • FunctionComponent of the MRE11-RAD50-NBN (MRN complex) which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. NBN modulate the DNA damage signal sensing by recruiting PI3/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. It can also recruit MRE11 and RAD50 to the proximity of DSBs by an interaction with the histone H2AX. NBN also functions in telomere length maintenance by generating the 3' overhang which serves as a primer for telomerase dependent telomere elongation. NBN is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBN is involved in G1 and G2 checkpoints. The roles of NBS1/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability. Forms a complex with RBBP8 to link DNA double-strand break sensing to resection. Enhances AKT1 phosphorylation possibly by association with the mTORC2 complex.
    • Tissue specificityUbiquitous. Expressed at high levels in testis.
    • Involvement in diseaseNijmegen breakage syndrome
      Breast cancer
      Aplastic anemia
      Defects in NBN might play a role in the pathogenesis of childhood acute lymphoblastic leukemia (ALL).
    • Sequence similaritiesContains 1 BRCT domain.
      Contains 1 FHA domain.
    • DomainThe FHA and BRCT domains are likely to have a crucial role for both binding to histone H2AFX and for relocalization of MRE11/RAD50 complex to the vicinity of DNA damage.
      The C-terminal domain contains a MRE11-binding site, and this interaction is required for the nuclear localization of the MRN complex.
      The EEXXXDDL motif at the C-terminus is required for the interaction with ATM and its recruitment to sites of DNA damage and promote the phosphorylation of ATM substrates, leading to the events of DNA damage response.
    • Post-translational
      modifications
      Phosphorylated by ATM in response of ionizing radiation, and such phosphorylation is responsible intra-S phase checkpoint control and telomere maintenance.
    • Cellular localizationNucleus. Nucleus, PML body. Chromosome, telomere. Localizes to discrete nuclear foci after treatment with genotoxic agents.
    • Information by UniProt
    • Database links
    • Alternative names
      • AT V1 antibody
      • AT V2 antibody
      • ATV antibody
      • Cell cycle regulatory protein p95 antibody
      • FLJ10155 antibody
      • MGC87362 antibody
      • Nbn antibody
      • NBN_HUMAN antibody
      • NBS 1 antibody
      • NBS antibody
      • NBS1 antibody
      • Nibrin antibody
      • Nijmegen breakage syndrome 1 (nibrin) antibody
      • Nijmegen breakage syndrome antibody
      • Nijmegen breakage syndrome protein 1 antibody
      • p95 antibody
      • p95 protein of the MRE11/RAD50 complex antibody
      see all

    Anti-p95 NBS1 (phospho S432) antibody [EPR2470Y] images

    • Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: p95 NBS1 (pS432) phospho peptide

      Lane 2 sample: p95 NBS1 non-phospho peptide

      Exposure time: 3 minutes

    • ab75778 staining p95 NBS1 (phospho S432) in Human testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/300). An undiluted HRP-conjugated mouse anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin. PBS was used in the negative control rather than the Primary antibody.

    • ab75778 at 1/100-1/250 dilution staining p95 NBS1 in human testis by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-p95 NBS1 (phospho S432) antibody [EPR2470Y] (ab75778)

    This product has been referenced in:
    • Kefala M  et al. Increased expression of phosphorylated NBS1, a key molecule of the DNA damage response machinery, is an adverse prognostic factor in patients with de novo myelodysplastic syndromes. Leuk Res 37:1576-82 (2013). Human . Read more (PubMed: 24054861) »
    • Cherubini G  et al. The FANC pathway is activated by adenovirus infection and promotes viral replication-dependent recombination. Nucleic Acids Res : (2011). WB ; Human . Read more (PubMed: 21421559) »

    See all 2 Publications for this product

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