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Anti-pCrosstide antibody (ab28546)

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Overview

Product name

Anti-pCrosstide antibody
See all pCrosstide products (2) ...

Description

Rabbit polyclonal to pCrosstide

Specificity

Recognize only the phophophorylated product of SGK. This antibody does not cross react with the non phosphorylated form of Crosstide.

Tested applications

ELISA, WBmore details

Immunogen

Synthetic peptide conjugated to KLH -PRAApTF

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: Tris buffered saline

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

This affinity purified rabbit polyclonal anti-pCrosstide was developed with phosphorylated Crosstide (PRAApTF) and absorbed with non-phosphorylated Crosstide (PRAATF).

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT

Applications

Show applications key

Our Abpromise guarantee covers the use of ab28546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     

  • WB

     

Application notes

ELISA: Use at a concentration of 0.25 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 1 kDa.
Non-radioactive AKT and SGK kinase assay.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

Crosstide is a synthetic peptide derived from the N terminal sequence of GSK3 and is a well documented substrate of PKB (AKT) and SGK kinases. pCrosstide is the phosphorylated product of these kinases.

Alternative names

  • PRAApTF antibody

References for Anti-pCrosstide antibody (ab28546)

ab28546 has not yet been referenced specifically in any publications.

Publishing research using ab28546? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"