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Product Name
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pCrosstide antibody
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See all pCrosstide antibodies (2)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to pCrosstide
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Immunogen
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Synthetic peptide conjugated to KLH -PRAApTF
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Reacts with
(species key)
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N/A
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Specificity
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Recognize only the phophophorylated product of SGK. This antibody does not cross react with the non phosphorylated form of Crosstide.
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.25 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 1 kDa.
Non-radioactive AKT and SGK kinase assay.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Research areas
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Chromatin and Nuclear Signaling >> Cell cycle >> Kinases/Phosphatases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKB / AKT
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Relevance
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Crosstide is a synthetic peptide derived from the N terminal sequence of GSK3 and is a well documented substrate of PKB (AKT) and SGK kinases. pCrosstide is the phosphorylated product of these kinases.
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.1% Sodium Azide
Constituents: TBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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This affinity purified rabbit polyclonal anti-pCrosstide was developed with phosphorylated Crosstide (PRAApTF) and absorbed with non-phosphorylated Crosstide (PRAATF).
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this pCrosstide antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to pCrosstide
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pCrosstide antibody - more information
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