Overview

  • Product name
  • Description
    Rabbit polyclonal to PAAF1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, between N-terminal amino acids 115-145 of Human PAAF1.

  • Positive control
    • A549 cell lysate and Human brain tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab103566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 42 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Inhibits proteasome 26S assembly and proteolytic activity by impairing the association of the 19S regulatory complex with the 20S core. In case of HIV-1 infection, recruited by viral Tat to the HIV-1 promoter, where it promotes the recruitment of 19S regulatory complex through dissociation of the proteasome 26S. This presumably promotes provirus transcription efficiency.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in kidney, brain and testis.
  • Sequence similarities
    Belongs to the WD repeat PAAF1/RPN14 family.
    Contains 5 WD repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • PAAF antibody
    • PAAF1 antibody
    • PAAF1_HUMAN antibody
    • Proteasomal ATPase-associated factor 1 antibody
    • Protein G-16 antibody
    • Rpn14 antibody
    • WD repeat domain 71 antibody
    • WD repeat-containing protein 71 antibody
    • WDR71 antibody
    see all

Images

  • Anti-PAAF1 antibody (ab103566) at 1/100 dilution + A549 cell lysate at 35 µg

    Predicted band size : 42 kDa
  • IHC analysis in formalin fixed and paraffin embedded Human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining, using ab103566 at a dilution of 1/50.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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