• Product nameAnti-PABP2 antibody [EP3001Y]
    See all PABP2 primary antibodies
  • Description
    Rabbit monoclonal [EP3001Y] to PABP2
  • Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Cow, Human
  • Immunogen

    Synthetic peptide from Human PABP2

  • Positive control
    • WB: T47D, SKBR3, Ramos and Raji cell lysates; IHC: urinary bladder transitional cell carcinoma.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab81224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
IHC-P 1/100 - 1/250.
ICC 1/100 - 1/250.
Flow Cyt 1/60. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for IP.
  • Target

    • RelevancePABP2 is involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation. Two different isoform have been identified.
    • Cellular localizationCytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Nuclear poly(A) binding protein 1 antibody
      • PAB2 antibody
      • PABII antibody
      • PABP2 antibody
      • PABPN1 antibody
      • Poly(A) binding protein 2 antibody
      • Polyadenylate binding nuclear protein 1 antibody
      • Polyadenylate binding protein 2 antibody
      • ROX2 antibody
      see all

    Anti-PABP2 antibody [EP3001Y] images

    • All lanes : Anti-PABP2 antibody [EP3001Y] (ab81224) at 1/20000 dilution

      Lane 1 : T47D cell lysates
      Lane 2 : SKBR3 cell lysates
      Lane 3 : Ramos cell lysates
      Lane 4 : Raji cell lysates

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 33 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry staining of paraffin-embedded urinary bladder transitional cell carcinoma tissue, using 1/100 ab81224
    • Overlay histogram showing HeLa cells stained with ab81224 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81224, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    References for Anti-PABP2 antibody [EP3001Y] (ab81224)

    This product has been referenced in:
    • Kim SH  et al. ALS-associated proteins TDP-43 and FUS/TLS function in a common biochemical complex to coregulate HDAC6 mRNA. J Biol Chem : (2010). WB ; Human . Read more (PubMed: 20720006) »
    • Kim SH  et al. Amyotrophic lateral sclerosis-associated proteins TDP-43 and FUS/TLS function in a common biochemical complex to co-regulate HDAC6 mRNA. J Biol Chem 285:34097-105 (2010). Read more (PubMed: 20720006) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 2e+006 cells
    Gel Running Conditions Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
    Sample Cow Cell lysate - whole cell (bovine PABPN1 expressed in yeast S. cerevisiae cel)
    Specification bovine PABPN1 expressed in yeast S. cerevisiae cel
    Blocking step (agent) for 30 minute(s) · Concentration: 5% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Jun 17 2013