Overview

  • Product nameAnti-PABPC3 antibody
    See all PABPC3 primary antibodies
  • Description
    Mouse polyclonal to PABPC3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    PABPC3 (1 a.a. ~ 631 a.a) full-length human protein.

  • Positive control
    • PABPC3 transfected 293T cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.4
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab68090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 70 kDa).

Target

  • FunctionBinds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Binds poly(A) with a slightly lower affinity as compared to PABPC1.
  • Tissue specificityTestis specific.
  • Sequence similaritiesBelongs to the polyadenylate-binding protein type-1 family.
    Contains 1 PABC domain.
    Contains 4 RRM (RNA recognition motif) domains.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • PABP-3 antibody
    • PABP3 antibody
    • PABP3_HUMAN antibody
    • PABPC3 antibody
    • PABPL3 antibody
    • Poly(A) binding protein 3 antibody
    • Poly(A)-binding protein 3 antibody
    • Polyadenylate binding protein 3 antibody
    • Polyadenylate-binding protein 3 antibody
    • RP11-165I9.5 antibody
    • Testis specific poly(A) binding protein antibody
    • Testis-specific poly(A)-binding protein antibody
    • tPABP antibody
    see all

Anti-PABPC3 antibody images

  • All lanes : Anti-PABPC3 antibody (ab68090) at 1/500 dilution

    Lane 1 : PABPC3 transfected 293T cell lysate
    Lane 2 : non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 70 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References for Anti-PABPC3 antibody (ab68090)

ab68090 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Testis)
Specification Testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Novocastra pH 6 retrieval buffer
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3µg/mL · Temperature: 22°C
Username

Dr. Aamir Ahmed

Verified customer

Submitted Jan 27 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"