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Anti-PABPN1 antibody [EP3000Y] (ab75855)

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Overview

Product name

Anti-PABPN1 antibody [EP3000Y]

Description

Rabbit monoclonal [EP3000Y] to PABPN1

Tested applications

ICC/IF, WB, IP, IHC-P, Flow Cytmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Rat

Immunogen

A synthetic peptide corresponding to residues near the N-terminus of human PABPN1

Positive control

Raw264.7, MCF-7 or HeLa cell lysates. Squamous cell cervical carcinoma tissue. HeLa cells.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP3000Y

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> RNAi >> P bodies/GW bodies
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Splicing

  • Western blot - PABPN1 antibody [EP3000Y] (ab75855)Western blot - PABPN1 antibody [EP3000Y] (ab75855) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABPN1 antibody [EP3000Y] (ab75855)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABPN1 antibody [EP3000Y] (ab75855) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - PABPN1 antibody [EP3000Y] (ab75855)Immunocytochemistry/ Immunofluorescence - PABPN1 antibody [EP3000Y] (ab75855) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Flow Cyt: 1/80.
ICC/IF: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
IP: 1/50.
WB: 1/100000 - 1/1000000. Predicted molecular weight: 33 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

PABPN1 is involved in the 3'end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. It stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. It increases the affinity of poly(A) polymerase for RNA. It is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. It cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. It binds to poly(A) and to poly(G) with high affinity. It may protect the poly(A) tail from degradation (referenced from swissprot).

Cellular localization

Shuttles between the nucleus and the cytoplasm, predominantly in the nucleus.

Alternative names

  • Nuclear poly(A)-binding protein 1 antibody
  • PAB2 antibody
  • PABII antibody
  • PABP2 antibody
  • PABPII antibody
  • poly(A) binding protein nuclear 1 antibody
  • PolyA binding protein II antibody
see all

Database links

Anti-PABPN1 antibody [EP3000Y] images:

  Western blot - PABPN1 antibody [EP3000Y] (ab75855)

Western blot - PABPN1 antibody [EP3000Y] (ab75855)

Lanes 1 - 2 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/200000 dilution
Lane 3 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/1000000 dilution

Lane 1 : Raw264.7 cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Goat anti-rabbit HRP at 1/1000 dilution

Predicted band size : 33 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABPN1 antibody [EP3000Y] (ab75855)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PABPN1 antibody [EP3000Y] (ab75855)

ab75855, at 1/100 dilution, staining PABPN1 in squamous cell cervical carcinoma, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

  Immunocytochemistry/ Immunofluorescence - PABPN1 antibody [EP3000Y] (ab75855)

Immunocytochemistry/ Immunofluorescence - PABPN1 antibody [EP3000Y] (ab75855)

ab75855, at 1/100 dilution, staining PABPN1 in HeLa cells, by Immunofluorescent staining.

  Flow Cytometry - Anti-PABPN1 antibody [EP3000Y] (ab75855)

Flow Cytometry - Anti-PABPN1 antibody [EP3000Y] (ab75855)

Overlay histogram showing MCF-7 cells stained with ab75855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75855, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-PABPN1 antibody [EP3000Y] (ab75855)

ab75855 has not yet been referenced specifically in any publications.

Publishing research using ab75855? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"