Anti-PABPN1 antibody [EP3000Y] (ab75855)


  • Product name
    Anti-PABPN1 antibody [EP3000Y]
    See all PABPN1 primary antibodies
  • Description
    Rabbit monoclonal [EP3000Y] to PABPN1
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PABPN1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Raw264.7, MCF-7 and HeLa cell lysates. ICC/IF: MCF-7 cells. Flow Cyt: MCF-7 cells. IHC-P: Squamous cell cervical carcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.


    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


    Alternative versions available:

    Anti-PABPN1 antibody (Alexa Fluor® 488) [EP3000Y] (ab206056)

    Anti-PABPN1 antibody (Alexa Fluor® 647) [EP3000Y] (ab206057)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab75855 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.


  • Function
    Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly(A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly(A) polymerase (PAPOLA) conferring processivity on the poly(A) tail elongation reaction and controls also the poly(A) tail length. Increases the affinity of poly(A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly(A) and to poly(G) with high affinity. May protect the poly(A) tail from degradation.
  • Tissue specificity
  • Involvement in disease
    Defects in PABPN1 are the cause of oculopharyngeal muscular dystrophy (OPMD) [MIM:164300]. OPMD is a form of late-onset slowly progressive myopathy characterized by eyelid ptosis, dysphagia and, sometimes by other cranial and limb-muscle involvement.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain
    The RRM domain is essential for specific adenine bases recognition in the poly(A) tail but not sufficient for poly(A) binding.
  • Post-translational
    Arginine dimethylation is asymmetric and involves PRMT1 and PRMT3. It does not influence the RNA binding properties.
  • Cellular localization
    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus. Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP-sensitive import mechanism. Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Nucleus; nuclear speckle. Colocalizes with SKIP and poly(A) RNA in nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Nuclear poly(A)-binding protein 1 antibody
    • OPMD antibody
    • PAB2 antibody
    • PABII antibody
    • PABP 2 antibody
    • pABP-2 antibody
    • PABP2 antibody
    • PABP2_HUMAN antibody
    • PABPII antibody
    • Pabpn1 antibody
    • poly(A) binding protein nuclear 1 antibody
    • Poly(A)-binding protein 2 antibody
    • Poly(A)-binding protein II antibody
    • PolyA binding protein II antibody
    • Polyadenylate-binding nuclear protein 1 antibody
    • Polyadenylate-binding protein 2 antibody
    see all

Anti-PABPN1 antibody [EP3000Y] images

  • Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling PABPN1 with ab75855 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Lanes 1 - 2 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/200000 dilution
    Lane 3 : Anti-PABPN1 antibody [EP3000Y] (ab75855) at 1/1000000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

    Predicted band size : 33 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing MCF-7 cells stained with ab75855 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75855, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab75855, at 1/100 dilution, staining PABPN1 in squamous cell cervical carcinoma, by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

References for Anti-PABPN1 antibody [EP3000Y] (ab75855)

This product has been referenced in:
  • Malerba A  et al. PABPN1 gene therapy for oculopharyngeal muscular dystrophy. Nat Commun 8:14848 (2017). WB, IHC ; Mouse . Read more (PubMed: 28361972) »
  • Klein P  et al. Nuclear poly(A)-binding protein aggregates misplace a pre-mRNA outside of SC35 speckle causing its abnormal splicing. Nucleic Acids Res 44:10929-10945 (2016). Read more (PubMed: 27507886) »

See all 12 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Human Cell lysate - whole cell (HEK 293T)
HEK 293T
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
Anti-hnRNP C antibody (sc-15386) bound protein G dynabeads, 0.1 mg/ml (antibody/beads)
Negative control
Protein G dynabeads without antibody bound
Immuno-precipitation step
Other - Protein G dynabeads

Abcam user community

Verified customer

Submitted Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (293T)
Loading amount
30000 cells
Gel Running Conditions
Reduced Denaturing (4-20% Tris-Glycine)
Blocking step
5% Milk, 1% BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 26 2010


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