Anti-PACAP receptor/ADCYAP1R1 antibody (ab95278)
Key features and details
- Rabbit polyclonal to PACAP receptor/ADCYAP1R1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PACAP receptor/ADCYAP1R1 antibody
See all PACAP receptor/ADCYAP1R1 primary antibodies -
Description
Rabbit polyclonal to PACAP receptor/ADCYAP1R1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Cow -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following tissue lysates: Human brain; Human liver; Human pancreas; Human small intestine.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
Target
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Function
This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract. -
Tissue specificity
Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 117 Human
- Omim: 102981 Human
- SwissProt: P41586 Human
- Unigene: 377783 Human
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Alternative names
- ADCYAP1 R1 antibody
- ADCYAP1R 1 antibody
- ADCYAP1R1 antibody
see all
Images
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All lanes : Anti-PACAP receptor/ADCYAP1R1 antibody (ab95278) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 51 kDa (possible cleavage fragment)
Exposure time: 10 minutesThe band observed at 51 kDa could potentially be a cleaved form of PACAP receptor/ADCYAP1R1 due to the presence of a 20 amino acid signal peptide. A positive signal was also seen in both Human liver and Human pancreas tissue lysates.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab95278 has been referenced in 1 publication.
- Krajcs N et al. PACAP Modulates Acetylcholine-Elicited Contractions at Nicotinic Neuromuscular Contacts of the Land Snail. J Mol Neurosci 57:492-500 (2015). PubMed: 26138333