The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
This is a receptor for PACAP-27 and PACAP-38. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. May regulate the release of adrenocorticotropin, luteinizing hormone, growth hormone, prolactin, epinephrine, and catecholamine. May play a role in spermatogenesis and sperm motility. Causes smooth muscle relaxation and secretion in the gastrointestinal tract.
Most abundant in the brain, low expression in the lung, liver, thymus, spleen, pancreas and placenta.
Belongs to the G-protein coupled receptor 2 family.
Pituitary adenylate cyclase activating polypeptide type I receptor precursor antibody
Pituitary adenylate cyclase-activating polypeptide 1, receptor for antibody
Pituitary adenylate cyclase-activating polypeptide type I receptor antibody
Western blot - PACAP receptor antibody (ab95278)
All lanes : Anti-PACAP receptor antibody (ab95278) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa Observed band size: 53 kDa Additional bands at: 51 kDa (possible cleavage fragment)
Exposure time: 10 minutes
The band observed at 51 kDa could potentially be a cleaved form of PACAP receptor due to the presence of a 20 amino acid signal peptide.
A positive signal was also seen in both Human liver and Human pancreas tissue lysates.