• Product nameAnti-PACE4 antibody
    See all PACE4 primary antibodies
  • Description
    Goat polyclonal to PACE4
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:


    , corresponding to internal sequence amino acids 830-841 of Human PACE4 (NP_002561.1; NP_612192.1; NP_612195.1; NP_612197.1; NP_612196.1; NP_612198.2; NP_612193.1; NP_612194.1).

  • Positive control
    • Human testis and skeletal muscle tissues



Our Abpromise guarantee covers the use of ab110144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. Fix cells in paraformaldehyde and permeabilize with 0.5% Triton X-100/PBS before staining.
IHC-P Use a concentration of 3.75 µg/ml.


  • FunctionLikely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
  • Tissue specificityEach PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
  • Sequence similaritiesBelongs to the peptidase S8 family.
    Contains 1 homo B/P domain.
    Contains 1 PLAC domain.
  • DomainThe propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
  • Cellular localizationSecreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Paired basic amino acid cleaving enzyme 4 antibody
    • Paired basic amino acid cleaving system 4 antibody
    • PCSK6 antibody
    • PCSK6_HUMAN antibody
    • Proprotein convertase subtilisin/kexin type 6 antibody
    • SPC4 antibody
    • Subtilisin like protease antibody
    • Subtilisin-like proprotein convertase 4 antibody
    • subtilisin/kexin like protease PACE4 antibody
    • Subtilisin/kexin-like protease PACE4 antibody
    see all

Anti-PACE4 antibody images

  • ab110144, at 3.75 µg/ml, staining PACE4 in formalin fixed, paraffin embedded Human skeletal muscle by Immunohistochemistry.
  • ab110144, at 3.75 µg/ml, staining PACE4 in formalin fixed, paraffin embedded Human testis by Immunohistochemistry.
  • ab110144 (1/200) staining PACE4 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References for Anti-PACE4 antibody (ab110144)

ab110144 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X100 in PBS

Dr. Kirk McManus

Verified customer

Submitted Nov 15 2011