Overview

  • Product name
  • Description
    Goat polyclonal to PACE4
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    PDCEPGTYFDSE

    , corresponding to internal sequence amino acids 830-841 of Human PACE4 (NP_002561.1; NP_612192.1; NP_612195.1; NP_612197.1; NP_612196.1; NP_612198.2; NP_612193.1; NP_612194.1).

  • Positive control
    • Human testis and skeletal muscle tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab110144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200. Fix cells in paraformaldehyde and permeabilize with 0.5% Triton X-100/PBS before staining.
IHC-P Use a concentration of 3.75 µg/ml.

Target

  • Function
    Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
  • Tissue specificity
    Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
  • Sequence similarities
    Belongs to the peptidase S8 family.
    Contains 1 homo B/P domain.
    Contains 1 PLAC domain.
  • Domain
    The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
  • Cellular localization
    Secreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Paired basic amino acid cleaving enzyme 4 antibody
    • Paired basic amino acid cleaving system 4 antibody
    • PCSK6 antibody
    • PCSK6_HUMAN antibody
    • Proprotein convertase subtilisin/kexin type 6 antibody
    • SPC4 antibody
    • Subtilisin like protease antibody
    • Subtilisin-like proprotein convertase 4 antibody
    • subtilisin/kexin like protease PACE4 antibody
    • Subtilisin/kexin-like protease PACE4 antibody
    see all

Images

  • ab110144, at 3.75 µg/ml, staining PACE4 in formalin fixed, paraffin embedded Human skeletal muscle by Immunohistochemistry.
  • ab110144, at 3.75 µg/ml, staining PACE4 in formalin fixed, paraffin embedded Human testis by Immunohistochemistry.
  • ab110144 (1/200) staining PACE4 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

    See Abreview

References

ab110144 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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