The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200. Fix cells in paraformaldehyde and permeabilize with 0.5% Triton X-100/PBS before staining.
Use a concentration of 3.75 µg/ml.
FunctionLikely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
Tissue specificityEach PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
Sequence similaritiesBelongs to the peptidase S8 family. Contains 1 homo B/P domain. Contains 1 PLAC domain.
DomainThe propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
Cellular localizationSecreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
ab110144, at 3.75 µg/ml, staining PACE4 in formalin fixed, paraffin embedded Human testis by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-PACE4 antibody (ab110144)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
ab110144 (1/200) staining PACE4 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.