Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Overview
Product name
Anti-PACE4 antibody - Carboxyterminal end
See all PACE4 products (5) ...
Description
Rabbit polyclonal to PACE4 - Carboxyterminal end
Specificity
Recognizes the Homo-b domain in human PACE4. his antibody also recognizes all but the shortest of the splice variants of PACE4.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide based on the carboxy end of the Homo-b domain in human PACE4.
(Peptide available as ab41418.)
Positive control
PACE4 is expressed at low levels in most tissues, but found in highest concentration in the anterior pituitary, controlled in part by thyroid hormone levels. Other tissues expressing PACE4 include cerebellum, pancreatic islet cells, liver and kidneys, spleen, thymus, and gut.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody has been peptide-affinity purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1,000 when using colorimetric substrates such as BCIP/NBT and 1/5,000 for chemiluminescent substrates. Predicted molecular weight: 106 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
Tissue specificity
Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 homo B/P domain.
Contains 1 PLAC domain.
Domain
The propeptide domain acts as an intramolecular chaperone assisting the folding of the zymogen within the endoplasmic reticulum. Isoform PACE4D lacks the propeptide domain.
Cellular localization
Secreted; Endomembrane system. Retained intracellularly probably through a hydrophobic cluster in their C-terminus and Endoplasmic reticulum. Not secreted, remains probably in zymogen form in endoplasmic reticulum.
Target information above from: UniProt accessionP29122
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- paired basic amino acid cleaving enzyme 4 antibody
- paired basic amino acid cleaving system 4 antibody
- Pcsk6 antibody
see all
Database links
- Entrez Gene: 5046 Human
- Omim: 167405 Human
- SwissProt: P29122 Human
- Unigene: 498494 Human
- Unigene: 665989 Human
Protocols
References for Anti-PACE4 antibody - Carboxyterminal end (ab28542)
ab28542 has not yet been referenced specifically in any publications.
Publishing research using ab28542? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"