The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 µg/ml for 2hrs. Predicted molecular weight: 105 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionCoat protein that is involved in the localization of trans-Golgi network (TGN) membrane proteins that contain acidic cluster sorting motifs. Controls the endosome-to-Golgi trafficking of furin and mannose-6-phosphate receptor by connecting the acidic-cluster-containing cytoplasmic domain of these molecules with the adapter-protein complex-1 (AP-1) of endosomal clathrin-coated membrane pits. Involved in HIV-1 nef-mediated removal of MHC-I from the cell surface to the TGN.
Involvement in diseaseMental retardation, autosomal dominant 17
Sequence similaritiesBelongs to the PACS family.
Cellular localizationGolgi apparatus, trans-Golgi network. Localizes in the perinuclear region, probably the TGN.
Phosphofurin acidic cluster sorting protein 1 antibody
Anti-PACS1 antibody images
Western blot - PACS1 antibody (ab56072)
Predicted band size : 105 kDa
Image from Yi L et al, J Biol Chem. 2010 Oct 1;285(40):30884-905. Epub 2010 Jul 9, Fig 4. DOI 10.1074/jbc.M110.135947
References for Anti-PACS1 antibody (ab56072)
This product has been referenced in:
Werneburg NW et al. Tumor necrosis factor-related apoptosis inducing ligand (TRAIL) induced lysosomal translocation of proapoptotic effectors is mediated by phosphofurin acidic cluster sorting protein-2 (PACS-2). J Biol Chem : (2012).
Read more (PubMed: 22645134) »
Yi L et al. HIV-1 Nef binds a subpopulation of MHC-I throughout its trafficking itinerary and down-regulates MHC-I by perturbing both anterograde and retrograde trafficking. J Biol Chem285:30884-905 (2010).
Read more (PubMed: 20622010) »