PACSIN1 overexpression 293T lysate (whole cell) (ab94237)

Overview

  • Product namePACSIN1 overexpression 293T lysate (whole cell)
  • Description
    PACSIN1 overexpression lysate
  • General notesab94237 is a 293T cell transfected lysate in which Human PACSIN1 has been transiently over-expressed using a pCMV-PACSIN1 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research areas
  • BackgroundFunction: May play a role in vesicle formation and transport. Tissue specificity: Highly expressed in brain and in a lesser extent in heart, pancreas and liver. Similarity: Belongs to the PACSIN family. Contains 1 FCH domain. Contains 1 SH3 domain. PTM: Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).

Associated products

Applications

Our Abpromise guarantee covers the use of ab94237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

PACSIN1 overexpression 293T lysate (whole cell) images

  • ab94237 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-PACSIN1 antibody (ab89604) at 1/500 dilution

    Lane 1 : PACSIN1 overexpression 293T lysate (whole cell) (ab94237)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for PACSIN1 overexpression 293T lysate (whole cell) (ab94237)

ab94237 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"