The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 10 µg/ml. as capture antibody.
FunctionMay play a role in vesicle formation and transport.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the PACSIN family. Contains 1 FCH domain. Contains 1 SH3 domain.
Post-translational modificationsPhosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).