Overview

  • Product nameAnti-PACSIN2 antibody
    See all PACSIN2 primary antibodies
  • Description
    Mouse monoclonal to PACSIN2
  • Tested applicationsSuitable for: WB, IHC-P, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 368-429 of Human PACSIN2 (NP_009160) with a proprietary tag.

  • Positive control
    • Recombinant Protein. Human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab118330 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 56 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Sandwich ELISA Use a concentration of 10 µg/ml. as capture antibody.

Target

  • FunctionMay play a role in vesicle formation and transport.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the PACSIN family.
    Contains 1 FCH domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
  • Cellular localizationCytoplasmic vesicle. Vesicle-like cytoplasmic distribution.
  • Information by UniProt
  • Database links
  • Alternative names
    • cytoplasmic phosphoprotein PACSIN2 antibody
    • OTTHUMP00000198281 antibody
    • OTTHUMP00000198282 antibody
    • OTTHUMP00000198283 antibody
    • OTTHUMP00000198284 antibody
    • PACN2_HUMAN antibody
    • Pacsin2 antibody
    • protein kinase C and casein kinase substrate in neurons 2 antibody
    • Protein kinase C and casein kinase substrate in neurons protein 2 antibody
    • SDPII antibody
    • Syndapin II antibody
    see all

Anti-PACSIN2 antibody images

  • Anti-PACSIN2 antibody (ab118330) at 5 µg/ml + Recombinant immunogen at 0.2 µg
    Developed using the ECL technique

    Predicted band size : 56 kDa
  • ab118330 at 3 µg/ml staining PACSIN2 in Formalin-Fixed, Paraffin-Embedded Human Kidney tissue by Immunohistochemistry.
  • ab118330 at 10 µg/ml as a capture antibody. The detection limit for recombinant tagged PACSIN2 is 0.1 ng/ml.

References for Anti-PACSIN2 antibody (ab118330)

ab118330 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"