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Rabbit polyclonal to PACSIN2
Synthetic peptide corresponding to Human PACSIN2 aa 342-371 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
WB: Mouse brain and heart tissue lysates. T-47D, Daudi and HL-60 cell lysates.
IHC-P: Human breast carcinoma tissue.
ICC/IF: HeLa cells.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Concentration information loading...
Ammonium Sulphate Precipitation
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 5 µg/ml.
1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
1/50 - 1/100.
May play a role in vesicle formation and transport.
Belongs to the PACSIN family.
Contains 1 FCH domain. Contains 1 SH3 domain.
Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
Cytoplasmic vesicle. Vesicle-like cytoplasmic distribution.
Information by UniProt
cytoplasmic phosphoprotein PACSIN2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PACSIN2 antibody (ab37615)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the primary antibody at 1/50, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
Western blot - Anti-PACSIN2 antibody (ab37615)
All lanes : Anti-PACSIN2 antibody (ab37615) Lane 1 : HL-60 cell lysate Lane 2 : Daudi cell lysate Lane 3 : Mouse brain tissue lysate Lane 4 : Mouse heart lysate Lysates/proteins at 35 µg per lane. Predicted band size: 56 kDa
Western blot - PACSIN2 antibody (ab37615)
All lanes : Anti-PACSIN2 antibody (ab37615) at 1/100 dilution Lane 1 : T-47D cell lysate Lane 2 : Mouse brain tissue lysate Predicted band size: 56 kDa Observed band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PACSIN2 antibody (ab37615)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PACSIN2 (green) with ab37615 at 1/100. An Alexa Fluor
® 488-conjugated goat anti-rabbit IgG (1/400) was used as the secondary antibody. Cytoplasmic actin was counterstained using a DyLight Fluor ® 554-conjugated Phalloidin (red).
Immunocytochemistry/ Immunofluorescence - PACSIN2 antibody (ab37615)
ICC/IF image of ab37615 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37615, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab37615 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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