Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab2226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000. This has only been tested against the recombinant protein.
WB Use a concentration of 0.3 µg/ml. Detects a band of approximately 50 kDa.Can be blocked with Human PACSIN3 peptide (ab22870).

Target

  • FunctionMay play a role in vesicle formation and transport.
  • Tissue specificityExpressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.
  • Sequence similaritiesBelongs to the PACSIN family.
    Contains 1 FCH domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
  • Cellular localizationCytoplasm. Cell membrane. Detected at the inner aspect of the plasma membrane in cells or myotubes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endophilin 1 antibody
    • Endophilin I antibody
    • FLJ17861 antibody
    • OTTHUMP00000234262 antibody
    • OTTHUMP00000234263 antibody
    • OTTHUMP00000234264 antibody
    • PACN3_HUMAN antibody
    • Pacsin3 antibody
    • Protein kinase C and casein kinase substrate in neurons 3 antibody
    • Protein kinase C and casein kinase substrate in neurons protein 3 antibody
    • SDPIII antibody
    • SH3 domain containing protein 6511 antibody
    • SH3 domain-containing protein 6511 antibody
    • syndapin III antibody
    see all

Anti-PACSIN3 antibody images

  • Western Blot staining for ab2226 (1µg/ml) of H460 lysate (35µg total protein per lane). Primary incubated for 1 hour.

References for Anti-PACSIN3 antibody (ab2226)

This product has been referenced in:
  • Roach W & Plomann M PACSIN3 overexpression increases adipocyte glucose transport through GLUT1. Biochem Biophys Res Commun 355:745-50 (2007). WB ; Human . Read more (PubMed: 17320047) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry and patience. Ab2226 was raised against the synthetic peptide APEEDAGGEALGGS, corresponding to amino acids 2-15 of Human PACSIN3. It is an affinity purified anti-peptide antibody and so shouldn't react with other regions. How...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"