Overview

  • Product nameAnti-PACSIN3 antibody
    See all PACSIN3 primary antibodies
  • Description
    Rabbit polyclonal to PACSIN3
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human PACSIN3 aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Mouse brain tissue lysate; Human breast carcinoma tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab37612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IHC-P 1/50 - 1/100.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionMay play a role in vesicle formation and transport.
  • Tissue specificityExpressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.
  • Sequence similaritiesBelongs to the PACSIN family.
    Contains 1 FCH domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
  • Cellular localizationCytoplasm. Cell membrane. Detected at the inner aspect of the plasma membrane in cells or myotubes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endophilin 1 antibody
    • Endophilin I antibody
    • FLJ17861 antibody
    • OTTHUMP00000234262 antibody
    • OTTHUMP00000234263 antibody
    • OTTHUMP00000234264 antibody
    • PACN3_HUMAN antibody
    • Pacsin3 antibody
    • Protein kinase C and casein kinase substrate in neurons 3 antibody
    • Protein kinase C and casein kinase substrate in neurons protein 3 antibody
    • SDPIII antibody
    • SH3 domain containing protein 6511 antibody
    • SH3 domain-containing protein 6511 antibody
    • syndapin III antibody
    see all

Anti-PACSIN3 antibody images

  • Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • Anti-PACSIN3 antibody (ab37612) + 293 cell lysate at 35 µg

    Predicted band size : 48 kDa
  • Anti-PACSIN3 antibody (ab37612) at 1/100 dilution + Mouse brain tissue lysate.

    Predicted band size : 48 kDa
    Observed band size : 48 kDa

References for Anti-PACSIN3 antibody (ab37612)

ab37612 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (kidney)
Specification kidney
Fixative Paraformaldehyde
Permeabilization Yes - 5% PBT
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Mr. SungHwa Shin

Verified customer

Submitted May 14 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"