Overview

  • Product name
  • Description
    Rabbit polyclonal to PACSIN3
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 325 and 375 of Human PACSIN3 (NP_057307.2)

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    May play a role in vesicle formation and transport.
  • Tissue specificity
    Expressed in heart and skeletal muscle, lung, kidney, adrenal gland and placenta.
  • Sequence similarities
    Belongs to the PACSIN family.
    Contains 1 FCH domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).
  • Cellular localization
    Cytoplasm. Cell membrane. Detected at the inner aspect of the plasma membrane in cells or myotubes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Endophilin 1 antibody
    • Endophilin I antibody
    • FLJ17861 antibody
    • OTTHUMP00000234262 antibody
    • OTTHUMP00000234263 antibody
    • OTTHUMP00000234264 antibody
    • PACN3_HUMAN antibody
    • Pacsin3 antibody
    • Protein kinase C and casein kinase substrate in neurons 3 antibody
    • Protein kinase C and casein kinase substrate in neurons protein 3 antibody
    • SDPIII antibody
    • SH3 domain containing protein 6511 antibody
    • SH3 domain-containing protein 6511 antibody
    • syndapin III antibody
    see all

Images

  • All lanes : Anti-PACSIN3 antibody (ab86077) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 47 kDa
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated using 3µg ab86077.
    20% of IP was loaded per lane, and probed with ab86077 at 1µg/ml (lane 1) or with a control IgG (lane 2).
    Detection: chemiluminescence with an exposure time of 3 seconds.

References

ab86077 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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