Anti-PACT (PKR activating protein) / PRKRA antibody (ab50916)


  • Product nameAnti-PACT (PKR activating protein) / PRKRA antibody
    See all PACT (PKR activating protein) / PRKRA primary antibodies
  • Description
    Rabbit polyclonal to PACT (PKR activating protein) / PRKRA
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: RLPEYTLSQE GGPAHKREYT TICRLESFME TGKGASKKQA KRNAAEKFLA, corresponding to amino acids 141-190 of Human PACT (PKR activating protein)/ PRKRA

  • Positive control
    • Jurkat cell lysate; human stomach tissue.



Our Abpromise guarantee covers the use of ab50916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.


  • FunctionActivates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.
  • Involvement in diseaseDefects in PRKRA are the cause of dystonia type 16 (DYT16) [MIM:612067]. DYT16 is an early-onset dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT16 patients have progressive, generalized dystonia with axial muscle involvement, oro-mandibular (sardonic smile) and laryngeal dystonia and, in some cases, parkinsonian features.
  • Sequence similaritiesBelongs to the PRKRA family.
    Contains 3 DRBM (double-stranded RNA-binding) domains.
  • DomainSelf-association may occur via interactions between DRBM domains as follows: DRBM 1/DRBM 1, DRBM 1/DRBM 2, DRBM 2/DRBM 2 or DRBM 3/DRBM3.
  • Post-translational
    Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • DYT16 antibody
    • HSD14 antibody
    • Interferon inducible double stranded RNA dependent protein kinase activator A antibody
    • interferon-inducible double stranded RNA-dependent activator antibody
    • Interferon-inducible double stranded RNA-dependent protein kinase activator A antibody
    • PACT antibody
    • PKR associated protein X antibody
    • PKR associating protein X antibody
    • PKR-associated protein X antibody
    • PKR-associating protein X antibody
    • PRKRA antibody
    • PRKRA_HUMAN antibody
    • Protein activator of the interferon induced protein kinase antibody
    • Protein activator of the interferon-induced protein kinase antibody
    • Protein kinase antibody
    • Protein kinase interferon inducible double stranded RNA dependent activator antibody
    • RAX antibody
    see all

Anti-PACT (PKR activating protein) / PRKRA antibody images

  • Anti-PACT (PKR activating protein) / PRKRA antibody (ab50916) at 1 µg/ml + Jurkat cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 34 kDa
  • ab50916 at a concentration of 4 µg/ml positively labelling epithelial cells of fundic gland (arrows) in paraffin-embedded human stomach tissue.

References for Anti-PACT (PKR activating protein) / PRKRA antibody (ab50916)

This product has been referenced in:
  • Yoshida K  et al. Interaction between PKR and PACT mediated by LPS-inducible NF-?B in human gingival cells. J Cell Biochem 113:165-73 (2012). WB . Read more (PubMed: 21882225) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab50916.
Please use the links above to contact us or submit feedback about this product.