Anti-PACT (PKR activating protein) / PRKRA antibody (ab77215)


  • Product nameAnti-PACT (PKR activating protein) / PRKRA antibody
    See all PACT (PKR activating protein) / PRKRA primary antibodies
  • Description
    Mouse monoclonal to PACT (PKR activating protein) / PRKRA
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 101-201 of Human PACT (PKR activating protein)/ PRKRA with a 26kDa tag

  • Positive control
    • PC12 cell lysate



Our Abpromise guarantee covers the use of ab77215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
ELISA Use at an assay dependent dilution. Detection limit is approximately 30ng/ml as a capture antibody when used against the immunogen.


  • FunctionActivates EIF2AK2/PKR in the absence of double stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1.
  • Involvement in diseaseDefects in PRKRA are the cause of dystonia type 16 (DYT16) [MIM:612067]. DYT16 is an early-onset dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYT16 patients have progressive, generalized dystonia with axial muscle involvement, oro-mandibular (sardonic smile) and laryngeal dystonia and, in some cases, parkinsonian features.
  • Sequence similaritiesBelongs to the PRKRA family.
    Contains 3 DRBM (double-stranded RNA-binding) domains.
  • DomainSelf-association may occur via interactions between DRBM domains as follows: DRBM 1/DRBM 1, DRBM 1/DRBM 2, DRBM 2/DRBM 2 or DRBM 3/DRBM3.
  • Post-translational
    Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.
  • Cellular localizationCytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • DYT16 antibody
    • HSD14 antibody
    • Interferon inducible double stranded RNA dependent protein kinase activator A antibody
    • interferon-inducible double stranded RNA-dependent activator antibody
    • Interferon-inducible double stranded RNA-dependent protein kinase activator A antibody
    • PACT antibody
    • PKR associated protein X antibody
    • PKR associating protein X antibody
    • PKR-associated protein X antibody
    • PKR-associating protein X antibody
    • PRKRA antibody
    • PRKRA_HUMAN antibody
    • Protein activator of the interferon induced protein kinase antibody
    • Protein activator of the interferon-induced protein kinase antibody
    • Protein kinase antibody
    • Protein kinase interferon inducible double stranded RNA dependent activator antibody
    • RAX antibody
    see all

Anti-PACT (PKR activating protein) / PRKRA antibody images

  • Anti-PACT (PKR activating protein) / PRKRA antibody (ab77215) at 5 µg/ml + PC12 cell lysate at 25 µg

    Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
    developed using the ECL technique

    Predicted band size : 34 kDa
    Observed band size : 34 kDa

References for Anti-PACT (PKR activating protein) / PRKRA antibody (ab77215)

ab77215 has not yet been referenced specifically in any publications.

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