Overview

  • Product name
    Anti-PADI1 / PAD1 antibody
    See all PADI1 / PAD1 primary antibodies
  • Description
    Rabbit polyclonal to PADI1 / PAD1
  • Specificity
    Reactivity with other PAD isoforms has not yet been determined.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 15 residue synthetic peptide derived from a domain specific for Human peptidylarginine deiminase type 1 protein.

  • Positive control
    • This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab24008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 75 kDa.
ICC/IF 1/1000.

Target

  • Function
    Catalyzes the deimination of arginine residues of proteins.
  • Tissue specificity
    Epidermis, prostate, testis, placenta, spleen and thymus.
  • Sequence similarities
    Belongs to the protein arginine deiminase family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • HPAD10 antibody
    • Padi1 antibody
    • PADI1_HUMAN antibody
    • PDI antibody
    • PDI1 antibody
    • Peptidylarginine deiminase I antibody
    • protein arginine deiminase type I antibody
    • Protein-arginine deiminase type I antibody
    • Protein-arginine deiminase type-1 antibody
    see all

Images

  • ab24008 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab24008 at 1/1000 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PADI1 / PAD1 antibody (ab24008) + Human epidermis

    Predicted band size : 75 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Zhang X  et al. Genome-Wide Analysis Reveals PADI4 Cooperates with Elk-1 to Activate c-Fos Expression in Breast Cancer Cells. PLoS Genet 7:e1002112 (2011). WB ; Human . Read more (PubMed: 21655091) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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