Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086)
Key features and details
- Mouse monoclonal [OTI4H5] to PADI4 / PAD4
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human, African green monkey
- Isotype: IgG2a
Overview
-
Product name
Anti-PADI4 / PAD4 antibody [OTI4H5]
See all PADI4 / PAD4 primary antibodies -
Description
Mouse monoclonal [OTI4H5] to PADI4 / PAD4 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein, corresponding to amino acids 1-663 of Human PADI4/ PAD4 produced in HEK293T cells (NP_036519).
-
Positive control
- HEK293T cells transfected with PADI4 / PAD4 , Human breast, Carcinoma of Human lung, Adenocarcinoma of Human ovary, Human pancreas, Adenocarcinoma of Human endometrium, Human prostate, Carcinoma of Human prostate, Carcinoma of Human bladder and Human lymphoma tissues, COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 , Hela and Jurkat cells
-
General notes
The clone number has been updated from 4H5 to OTI4H5, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
-
Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI4H5 -
Isotype
IgG2a -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000. Predicted molecular weight: 74 kDa.
|
|
IHC-P | (1) |
1/150.
|
ICC/IF |
1/100.
|
|
Flow Cyt (Intra) |
1/100.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
---|
WB
1/2000. Predicted molecular weight: 74 kDa. |
IHC-P
1/150. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. -
Tissue specificity
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. -
Involvement in disease
Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. -
Sequence similarities
Belongs to the protein arginine deiminase family. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils. - Information by UniProt
-
Database links
- Entrez Gene: 23569 Human
- Omim: 605347 Human
- SwissProt: Q9UM07 Human
- Unigene: 522969 Human
-
Alternative names
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
see all
Images
-
All lanes : Anti-PADI4 / PAD4 antibody [OTI4H5] (ab128086) at 1/2000 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY PADI4 / PAD4 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 74 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC206501) -
ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human breast tissue by Immunohistochemistry.
-
ab128086, at a dilution of 1/100, staining COS7 cells transiently transfected by pCMV6-ENTRY PADI4 / PAD4 by Immunofluorescence.
-
ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in HeLa cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometry (Intracellular).
-
ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded carcinoma of Human lung tissue by Immunohistochemistry.
-
ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human ovary tissue by Immunohistochemistry.
-
ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
-
ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Adenocarcinoma of Human endometrium tissue by Immunohistochemistry.
-
ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human prostate tissue by Immunohistochemistry.
-
ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human prostate tissue by Immunohistochemistry.
-
ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Carcinoma of Human bladder tissue by Immunohistochemistry.
-
ab128086, at a dilution of 1/150, staining PADI4 / PAD4 in paraffin-embedded Human lymphoma tissue by Immunohistochemistry.
-
ab128086, at a dilution of 1/100, staining PADI4 / PAD4 in Jurkat cells (Red), compared to a nonspecific negative control antibody (Blue), analysed by Flow cytometry (Intracellular).
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (31)
ab128086 has been referenced in 31 publications.
- Gijon M et al. The Clinical and Prognostic Significance of Protein Arginine Deiminases 2 and 4 in Colorectal Cancer. Pathobiology 89:38-48 (2022). PubMed: 34569542
- Chang XT et al. PADI4 promotes epithelial-mesenchymal transition(EMT) in gastric cancer via the upregulation of interleukin 8. BMC Gastroenterol 22:25 (2022). PubMed: 35045833
- Pasquero S et al. Novel antiviral activity of PAD inhibitors against human beta-coronaviruses HCoV-OC43 and SARS-CoV-2. Antiviral Res 200:105278 (2022). PubMed: 35288208
- Chen X et al. Inhibition of NETosis by a Nuclear-Penetrating Anti-DNA Autoantibody. Immunohorizons 6:356-365 (2022). PubMed: 35697479
- Hawez A et al. MiR-155 regulates neutrophil extracellular trap formation and lung injury in abdominal sepsis. J Leukoc Biol N/A:N/A (2021). PubMed: 34114683